doi: 10.1016/0014-5793(92)80236-a.
Matrix assembly of recombinant fibronectin polypeptide consisting of amino-terminal 70 kDa and carboxyl-terminal 37 kDa regions
Affiliations
- PMID: 1544488
- DOI: 10.1016/0014-5793(92)80236-a
Item in Clipboard
Matrix assembly of recombinant fibronectin polypeptide consisting of amino-terminal 70 kDa and carboxyl-terminal 37 kDa regions
FEBS Lett.
.
Free article
Abstract
Three different forms of recombinant human fibronectin polypeptides consisting of the amino-terminal 70 kDa region, the carboxyl-terminal 37 kDa region, or both, were expressed in mouse L cells. Although either the amino-terminal or the carboxyl-terminal region alone was only poorly incorporated into the extracellular matrix, the fused form of the polypeptide was highly capable of assembling into the matrix. These results indicate that matrix assembly of fibronectin requires both regions and can proceed in the absence of the type III repeats including the one containing the cell adhesive Arg-Gly-Asp sequence.
Similar articles
-
Fibronectin's amino-terminal matrix assembly site is located within the 29-kDa amino-terminal domain containing five type I repeats.J Biol Chem. 1988 Dec 25;263(36):19602-9. J Biol Chem. 1988. PMID: 2974038
-
Assembly of amino-terminal fibronectin dimers into the extracellular matrix.J Biol Chem. 1994 Jun 24;269(25):17192-8. J Biol Chem. 1994. PMID: 8006027
-
Role of the carboxyl-terminal Fib2 domain in fibronectin matrix assembly.J Cell Sci. 1995 Mar;108 ( Pt 3):907-15. doi: 10.1242/jcs.108.3.907. J Cell Sci. 1995. PMID: 7622619
-
Cell surface molecules that bind fibronectin's matrix assembly domain.J Biol Chem. 1991 May 25;266(15):9697-702. J Biol Chem. 1991. PMID: 1903394
-
Integrins in cell adhesion and signaling.Hum Cell. 1996 Sep;9(3):181-6. Hum Cell. 1996. PMID: 9183647 Review.
Cited by
-
Fibronectin Conformation and Assembly: Analysis of Fibronectin Deletion Mutants and Fibronectin Glomerulopathy (GFND) Mutants.Biochemistry. 2017 Aug 29;56(34):4584-4591. doi: 10.1021/acs.biochem.7b00589. Epub 2017 Aug 11. Biochemistry. 2017. PMID: 28745050 Free PMC article.
-
Assembly of fibronectin extracellular matrix.Annu Rev Cell Dev Biol. 2010;26:397-419. doi: 10.1146/annurev-cellbio-100109-104020. Annu Rev Cell Dev Biol. 2010. PMID: 20690820 Free PMC article. Review.
-
A novel role for the integrin-binding III-10 module in fibronectin matrix assembly.J Cell Biol. 1996 Apr;133(2):431-44. doi: 10.1083/jcb.133.2.431. J Cell Biol. 1996. PMID: 8609174 Free PMC article.
-
Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein.Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2153-8. doi: 10.1073/pnas.96.5.2153. Proc Natl Acad Sci U S A. 1999. PMID: 10051610 Free PMC article.
-
The Extracellular Matrix Environment of Clear Cell Renal Cell Carcinoma.Cancers (Basel). 2022 Aug 23;14(17):4072. doi: 10.3390/cancers14174072. Cancers (Basel). 2022. PMID: 36077607 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
