Telomere length control and transcriptional regulation of subtelomeric adhesins in Candida glabrata
- PMID: 15686568
- DOI: 10.1111/j.1365-2958.2004.04465.x
Telomere length control and transcriptional regulation of subtelomeric adhesins in Candida glabrata
Abstract
The pathogenic yeast Candida glabrata is able to bind in vitro to human epithelial cells. This interaction depends on expression of the adhesin Epa1p. The genome contains a number of EPA1 paralogues which localize to the subtelomeric regions of the C. glabrata. We have identified three hyperadherent mutants of C. glabrata. The first has an insertion adjacent to EPA7, an EPA1-related adhesin. The others disrupt the SIR3 and RIF1 genes of C. glabrata. We show that SIR3 and RIF1 are required for subtelomeric silencing in C. glabrata and that RIF1 regulates telomere length in C. glabrata. We show that the hyperadherent phenotype of the sir3Delta and rif1Delta deletion strains depends primarily on derepression of two novel members of the EPA gene family -EPA6 and EPA7. The sir3Delta and rif1Delta mutants show increased colonization of the kidney in a murine model of disseminated infection and this hypercolonization depends, at least in part, on derepression of EPA6 and EPA7. The analysis here is the first evidence that multiple EPA genes encode adhesins and demonstrates that transcription of at least two of these adhesins is regulated by subtelomeric silencing.
Similar articles
-
Molecular characterization of the silencing complex SIR in Candida glabrata hyperadherent clinical isolates.Fungal Genet Biol. 2018 Sep;118:21-31. doi: 10.1016/j.fgb.2018.05.005. Epub 2018 May 29. Fungal Genet Biol. 2018. PMID: 29857197
-
The Yak1p kinase controls expression of adhesins and biofilm formation in Candida glabrata in a Sir4p-dependent pathway.Mol Microbiol. 2005 Feb;55(4):1259-71. doi: 10.1111/j.1365-2958.2004.04475.x. Mol Microbiol. 2005. PMID: 15686569
-
yKu70/yKu80 and Rif1 regulate silencing differentially at telomeres in Candida glabrata.Eukaryot Cell. 2008 Dec;7(12):2168-78. doi: 10.1128/EC.00228-08. Epub 2008 Oct 3. Eukaryot Cell. 2008. PMID: 18836091 Free PMC article.
-
The mating type-like loci of Candida glabrata.Rev Iberoam Micol. 2014 Jan-Mar;31(1):30-4. doi: 10.1016/j.riam.2013.09.016. Epub 2013 Nov 16. Rev Iberoam Micol. 2014. PMID: 24252826 Review.
-
A yeast by any other name: Candida glabrata and its interaction with the host.Curr Opin Microbiol. 2005 Aug;8(4):378-84. doi: 10.1016/j.mib.2005.06.012. Curr Opin Microbiol. 2005. PMID: 15996895 Review.
Cited by
-
Chromosome-level assemblies from diverse clades reveal limited structural and gene content variation in the genome of Candida glabrata.BMC Biol. 2022 Oct 8;20(1):226. doi: 10.1186/s12915-022-01412-1. BMC Biol. 2022. PMID: 36209154 Free PMC article.
-
The role of Rif1 in telomere length regulation is separable from its role in origin firing.Elife. 2020 Jun 29;9:e58066. doi: 10.7554/eLife.58066. Elife. 2020. PMID: 32597753 Free PMC article.
-
Candida albicans Hyr1p confers resistance to neutrophil killing and is a potential vaccine _target.J Infect Dis. 2010 Jun 1;201(11):1718-28. doi: 10.1086/652407. J Infect Dis. 2010. PMID: 20415594 Free PMC article.
-
Megasatellites: a new class of large tandem repeats discovered in the pathogenic yeast Candida glabrata.Cell Mol Life Sci. 2010 Mar;67(5):671-6. doi: 10.1007/s00018-009-0216-y. Epub 2009 Nov 28. Cell Mol Life Sci. 2010. PMID: 19946728 Free PMC article. Review.
-
A newly discovered role of telomeres in an ancient organism.Nucleus. 2010 May-Jun;1(3):260-3. doi: 10.4161/nucl.1.3.11742. Epub 2010 Mar 3. Nucleus. 2010. PMID: 21327073 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous
