Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Feb;31(2):155-60.
doi: 10.1007/s10858-004-7912-6.

Probing protein-peptide binding surfaces using charged stable free radicals and transverse paramagnetic relaxation enhancement (PRE)

Michaël L Deschamps  1 , Ewa S Pilka, Jennifer R Potts, Iain D Campbell, Jonathan Boyd
Affiliations

Probing protein-peptide binding surfaces using charged stable free radicals and transverse paramagnetic relaxation enhancement (PRE)

Michaël L Deschamps et al. J Biomol NMR. 2005 Feb.

Abstract

Nitroxide species, which have an unpaired electron localized on a nitrogen atom, can be useful as NMR probes to identify areas of the surface of a protein involved in the formation of a complex. The proximity of an electron spin leads to higher NMR relaxation rates for protein nuclei. If a protein-ligand complex is formed the radical is excluded from certain sites on the protein surface, protecting them from relaxation effects. We show here that charged nitroxide species can be helpful for identifying regions of the surface of the 4F1(5)F1 module pair from human fibronectin involved in peptide binding.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. J Mol Biol. 1994 Jan 28;235(4):1302-11 - PubMed
    1. J Magn Reson. 2002 Aug;157(2):181-9 - PubMed
    1. Biophys J. 1999 Jul;77(1):443-53 - PubMed
    1. Biochemistry. 2002 Jan 29;41(4):1123-8 - PubMed
    1. Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12765-70 - PubMed

Publication types

LinkOut - more resources

  NODES
twitter 2