Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration
- PMID: 16386413
- DOI: 10.1016/j.matbio.2005.11.003
Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration
Abstract
The ability to generate tissue-specific ablation of gene expression has been extremely useful in connective tissue biology, as it can potentially overcome the early embryonic lethal phenotype often associated with universal gene knockout. The value of tissue-specific knockouts can be enhanced by also allowing gene ablation to occur at specific times during development, growth or aging. In the present work a transgenic mouse has been generated in which expression of Cre recombinase is under control of both the type II collagen promoter to allow cartilage-specific expression and a doxycycline response element to permit temporal control of expression. This mouse has been crossed with the Rosa26R reporter mouse, which possesses a floxed repressor element associated with a lacZ transgene, in order to validate the functional efficacy of the conditionally expressed Cre. The results demonstrate that excision of the floxed element can be achieved specifically in cartilage at different times during embryonic and juvenile development. The conditional Cre transgenic mouse should be a valuable tool to all interested in skeletal development.
Similar articles
-
Endothelium-specific Cre recombinase activity in flk-1-Cre transgenic mice.Dev Dyn. 2004 Feb;229(2):312-8. doi: 10.1002/dvdy.10416. Dev Dyn. 2004. PMID: 14745955
-
Mouse alpha1(I)-collagen promoter is the best known promoter to drive efficient Cre recombinase expression in osteoblast.Dev Dyn. 2002 Jun;224(2):245-51. doi: 10.1002/dvdy.10100. Dev Dyn. 2002. PMID: 12112477
-
Podocyte cell-specific expression of doxycycline inducible Cre recombinase in mice.J Am Soc Nephrol. 2006 Mar;17(3):648-54. doi: 10.1681/ASN.2005050547. Epub 2006 Feb 8. J Am Soc Nephrol. 2006. PMID: 16467448
-
Ocular surface tissue morphogenesis in normal and disease states revealed by genetically modified mice.Cornea. 2006 Dec;25(10 Suppl 1):S7-S19. doi: 10.1097/01.ico.0000247207.55520.a4. Cornea. 2006. PMID: 17001198 Review.
-
Conditional gene _targeting in the mouse nervous system: Insights into brain function and diseases.Pharmacol Ther. 2007 Mar;113(3):619-34. doi: 10.1016/j.pharmthera.2006.12.003. Epub 2007 Jan 10. Pharmacol Ther. 2007. PMID: 17289150 Review.
Cited by
-
Cell cycle deregulation and mosaic loss of Ext1 drive peripheral chondrosarcomagenesis in the mouse and reveal an intrinsic cilia deficiency.J Pathol. 2015 Jun;236(2):210-8. doi: 10.1002/path.4510. Epub 2015 Mar 3. J Pathol. 2015. PMID: 25644707 Free PMC article.
-
A mouse model of osteochondromagenesis from clonal inactivation of Ext1 in chondrocytes.Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2054-9. doi: 10.1073/pnas.0910875107. Epub 2009 Dec 22. Proc Natl Acad Sci U S A. 2010. PMID: 20080592 Free PMC article.
-
PPARγ deficiency results in severe, accelerated osteoarthritis associated with aberrant mTOR signalling in the articular cartilage.Ann Rheum Dis. 2015 Mar;74(3):569-78. doi: 10.1136/annrheumdis-2014-205743. Epub 2015 Jan 8. Ann Rheum Dis. 2015. PMID: 25573665 Free PMC article.
-
Modulating hedgehog signaling can attenuate the severity of osteoarthritis.Nat Med. 2009 Dec;15(12):1421-5. doi: 10.1038/nm.2055. Epub 2009 Nov 15. Nat Med. 2009. PMID: 19915594
-
The postnatal role of Sox9 in cartilage.J Bone Miner Res. 2012 Dec;27(12):2511-25. doi: 10.1002/jbmr.1696. J Bone Miner Res. 2012. PMID: 22777888 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
