Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
- PMID: 16904174
- DOI: 10.1016/j.cell.2006.07.024
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
Abstract
Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
Comment in
-
A transcriptional logic for nuclear reprogramming.Cell. 2006 Aug 25;126(4):652-5. doi: 10.1016/j.cell.2006.08.009. Cell. 2006. PMID: 16923385
-
Achieving pluripotency.Nat Rev Mol Cell Biol. 2010 Oct;11(10):677. doi: 10.1038/nrm2980. Nat Rev Mol Cell Biol. 2010. PMID: 20861870 No abstract available.
-
Literature Watch: implications for transplantation.Am J Transplant. 2013 Jun;13(6):1377. doi: 10.1111/ajt.12322. Am J Transplant. 2013. PMID: 23721549 No abstract available.
-
Induced pluripotent stem cells, past and future.Science. 2016 Sep 2;353(6303):991-992. doi: 10.1126/science.aai7626. Science. 2016. PMID: 27701103 Free PMC article.
-
Stem cells to help the heart.Science. 2020 Mar 13;367(6483):1206. doi: 10.1126/science.aba6111. Science. 2020. PMID: 32165579 No abstract available.
Similar articles
-
The timing of retroviral silencing correlates with the quality of induced pluripotent stem cell lines.Biochim Biophys Acta. 2011 Feb;1810(2):226-35. doi: 10.1016/j.bbagen.2010.10.004. Epub 2010 Oct 20. Biochim Biophys Acta. 2011. PMID: 20965232
-
Reprogramming of human somatic cells to pluripotency with defined factors.Nature. 2008 Jan 10;451(7175):141-6. doi: 10.1038/nature06534. Epub 2007 Dec 23. Nature. 2008. PMID: 18157115
-
Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells.Nat Biotechnol. 2007 Oct;25(10):1177-81. doi: 10.1038/nbt1335. Epub 2007 Aug 27. Nat Biotechnol. 2007. PMID: 17724450
-
Pluripotent genes in avian stem cells.Dev Growth Differ. 2013 Jan;55(1):41-51. doi: 10.1111/dgd.12021. Epub 2012 Dec 20. Dev Growth Differ. 2013. PMID: 23278808 Review.
-
Generation of clinically relevant "induced pluripotent stem" (iPS) cells.J Stem Cells. 2011;6(3):109-27. J Stem Cells. 2011. PMID: 23264997 Review.
Cited by
-
Generation and characterization of iPSC-derived renal proximal tubule-like cells with extended stability.Sci Rep. 2021 Jun 2;11(1):11575. doi: 10.1038/s41598-021-89550-4. Sci Rep. 2021. PMID: 34078926 Free PMC article.
-
Association of gankyrin and stemness factor expression in human colorectal cancer.Dig Dis Sci. 2013 Aug;58(8):2337-44. doi: 10.1007/s10620-013-2627-8. Epub 2013 Mar 19. Dig Dis Sci. 2013. PMID: 23508981
-
Stem cells in pediatric cardiology.Eur J Pediatr. 2013 Oct;172(10):1287-92. doi: 10.1007/s00431-012-1920-4. Epub 2013 Jan 5. Eur J Pediatr. 2013. PMID: 23292032 Review.
-
Calcium channels and their role in regenerative medicine.World J Stem Cells. 2021 Apr 26;13(4):260-280. doi: 10.4252/wjsc.v13.i4.260. World J Stem Cells. 2021. PMID: 33959218 Free PMC article. Review.
-
Reprogramming of cell fate: epigenetic memory and the erasure of memories past.EMBO J. 2015 May 12;34(10):1296-308. doi: 10.15252/embj.201490649. Epub 2015 Mar 27. EMBO J. 2015. PMID: 25820261 Free PMC article. Review.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous
