doi: 10.1038/nprot.2006.478.
Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts
Affiliations
- PMID: 17406588
- DOI: 10.1038/nprot.2006.478
Item in Clipboard
Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts
Nat Protoc.
2007.
Abstract
Mitochondria participate in key metabolic reactions of the cell and regulate crucial signaling pathways including apoptosis. Although several approaches are available to study mitochondrial function in situ are available, investigating functional mitochondria that have been isolated from different tissues and from cultured cells offers still more unmatched advantages. This protocol illustrates a step-by-step procedure to obtain functional mitochondria with high yield from cells grown in culture, liver and muscle. The isolation procedures described here require 1-2 hours, depending on the source of the organelles. The polarographic analysis can be completed in 1 hour.
Similar articles
-
Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues.Methods Mol Biol. 2017;1567:15-32. doi: 10.1007/978-1-4939-6824-4_2. Methods Mol Biol. 2017. PMID: 28276010
-
Isolation of Mitochondria from Murine Skeletal Muscle.Methods Mol Biol. 2024;2816:77-85. doi: 10.1007/978-1-0716-3902-3_8. Methods Mol Biol. 2024. PMID: 38977590
-
Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells.Nat Protoc. 2009;4(11):1582-90. doi: 10.1038/nprot.2009.151. Epub 2009 Oct 8. Nat Protoc. 2009. PMID: 19816421
-
Mitochondria: isolation, structure and function.J Physiol. 2011 Sep 15;589(Pt 18):4413-21. doi: 10.1113/jphysiol.2011.212712. Epub 2011 Jun 27. J Physiol. 2011. PMID: 21708903 Free PMC article. Review.
-
Isolation and subfractionation of mitochondria from animal cells and tissue culture lines.Methods Cell Biol. 2007;80:3-44. doi: 10.1016/S0091-679X(06)80001-4. Methods Cell Biol. 2007. PMID: 17445687 Review. No abstract available.
Cited by
-
Low levels of lipopolysaccharide modulate mitochondrial oxygen consumption in skeletal muscle.Metabolism. 2015 Mar;64(3):416-27. doi: 10.1016/j.metabol.2014.11.007. Epub 2014 Nov 28. Metabolism. 2015. PMID: 25528444 Free PMC article.
-
Bax regulates neuronal Ca2+ homeostasis.J Neurosci. 2015 Jan 28;35(4):1706-22. doi: 10.1523/JNEUROSCI.2453-14.2015. J Neurosci. 2015. PMID: 25632145 Free PMC article.
-
Pharmacologically _targetable vulnerability in prostate cancer carrying RB1-SUCLA2 deletion.Oncogene. 2020 Aug;39(34):5690-5707. doi: 10.1038/s41388-020-1381-6. Epub 2020 Jul 21. Oncogene. 2020. PMID: 32694611
-
Top down proteomics of human membrane proteins from enriched mitochondrial fractions.Anal Chem. 2013 Feb 5;85(3):1880-8. doi: 10.1021/ac3031527. Epub 2013 Jan 23. Anal Chem. 2013. PMID: 23305238 Free PMC article.
-
Berberine improves intralipid-induced insulin resistance in murine.Acta Pharmacol Sin. 2021 May;42(5):735-743. doi: 10.1038/s41401-020-0493-4. Epub 2020 Aug 7. Acta Pharmacol Sin. 2021. PMID: 32770172 Free PMC article.
Publication types
MeSH terms
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
