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. 1992 Feb 6;355(6360):564-6.
doi: 10.1038/355564a0.

Selection of single-stranded DNA molecules that bind and inhibit human thrombin

L C Bock  1 , L C Griffin, J A Latham, E H Vermaas, J J Toole
Affiliations

Selection of single-stranded DNA molecules that bind and inhibit human thrombin

L C Bock et al. Nature. .

Abstract

Aptamers are double-stranded DNA or single-stranded RNA molecules that bind specific molecular _targets. Large randomly generated populations can be enriched in aptamers by in vitro selection and polymerase chain reaction. But so far single-stranded DNA has not been investigated for aptamer properties, nor has a _target protein been considered that does not interact physiologically with nucleic acid. Here we describe the isolation of single-stranded DNA aptamers to the protease thrombin of the blood coagulation cascade and report binding affinities in the range 25-200 nM. Sequence data from 32 thrombin aptamers, selected from a pool of DNA containing 60 nucleotides of random sequence, displayed a highly conserved 14-17-base region. Several of these aptamers at nanomolar concentrations inhibited thrombin-catalysed fibrin-clot formation in vitro using either purified fibrinogen or human plasma.

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