Versatile, fully automated, microfluidic cell culture system
- PMID: 17953452
- DOI: 10.1021/ac071311w
Versatile, fully automated, microfluidic cell culture system
Abstract
There is increasing demand for automated and quantitative cell culture technology, driven both by the intense activity in stem cell biology and by the emergence of systems biology. We built a fully automated cell culture screening system based on a microfluidic chip that creates arbitrary culture media formulations in 96 independent culture chambers and maintains cell viability for weeks. Individual culture conditions are customized in terms of cell seeding density, composition of culture medium, and feeding schedule, and each chamber is imaged with time-lapse microscopy. Using this device, we perform the first quantitative measurements of the influence of transient stimulation schedules on the proliferation, osteogenic differentiation, and motility of human primary mesenchymal stem cells.
Similar articles
-
A gel-free 3D microfluidic cell culture system.Biomaterials. 2008 Aug;29(22):3237-44. doi: 10.1016/j.biomaterials.2008.04.022. Epub 2008 May 2. Biomaterials. 2008. PMID: 18455231
-
The culture and differentiation of amniotic stem cells using a microfluidic system.Biomed Microdevices. 2009 Aug;11(4):869-81. doi: 10.1007/s10544-009-9304-x. Biomed Microdevices. 2009. PMID: 19370418
-
Automated maintenance of embryonic stem cell cultures.Biotechnol Bioeng. 2007 Jan 1;96(1):195-201. doi: 10.1002/bit.21061. Biotechnol Bioeng. 2007. PMID: 16960892
-
Microfluidics for flow cytometric analysis of cells and particles.Physiol Meas. 2005 Jun;26(3):R73-98. doi: 10.1088/0967-3334/26/3/R02. Epub 2005 Feb 1. Physiol Meas. 2005. PMID: 15798290 Review.
-
Cells in microfluidics.Top Curr Chem. 2011;304:295-321. doi: 10.1007/128_2011_147. Top Curr Chem. 2011. PMID: 21598103 Review.
Cited by
-
Bioreactor engineering of stem cell environments.Biotechnol Adv. 2013 Nov 15;31(7):1020-31. doi: 10.1016/j.biotechadv.2013.03.007. Epub 2013 Mar 24. Biotechnol Adv. 2013. PMID: 23531529 Free PMC article. Review.
-
Highly parallelized human embryonic stem cell differentiation to cardiac mesoderm in nanoliter chambers on a microfluidic chip.Biomed Microdevices. 2021 May 31;23(2):30. doi: 10.1007/s10544-021-00556-1. Biomed Microdevices. 2021. PMID: 34059973 Free PMC article.
-
Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids.Nat Commun. 2020 Oct 19;11(1):5271. doi: 10.1038/s41467-020-19058-4. Nat Commun. 2020. PMID: 33077832 Free PMC article.
-
Probing embryonic stem cell autocrine and paracrine signaling using microfluidics.Annu Rev Anal Chem (Palo Alto Calif). 2012;5:293-315. doi: 10.1146/annurev-anchem-062011-143122. Epub 2012 Apr 9. Annu Rev Anal Chem (Palo Alto Calif). 2012. PMID: 22524217 Free PMC article. Review.
-
Single-cell NF-kappaB dynamics reveal digital activation and analogue information processing.Nature. 2010 Jul 8;466(7303):267-71. doi: 10.1038/nature09145. Epub 2010 Jun 27. Nature. 2010. PMID: 20581820 Free PMC article.
Publication types
MeSH terms
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
