PRC1 and Suv39h specify parental asymmetry at constitutive heterochromatin in early mouse embryos
- PMID: 18311137
- DOI: 10.1038/ng.99
PRC1 and Suv39h specify parental asymmetry at constitutive heterochromatin in early mouse embryos
Abstract
In eukaryotes, Suv39h H3K9 trimethyltransferases are required for pericentric heterochromatin formation and function. In early mouse preimplantation embryos, however, paternal pericentric heterochromatin lacks Suv39h-mediated H3K9me3 and downstream marks. Here we demonstrate Ezh2-independent _targeting of maternally provided polycomb repressive complex 1 (PRC1) components to paternal heterochromatin. In Suv39h2 maternally deficient zygotes, PRC1 also associates with maternal heterochromatin lacking H3K9me3, thereby revealing hierarchy between repressive pathways. In Rnf2 maternally deficient zygotes, the PRC1 complex is disrupted, and levels of pericentric major satellite transcripts are increased at the paternal but not the maternal genome. We conclude that in early embryos, Suv39h-mediated H3K9me3 constitutes the dominant maternal transgenerational signal for pericentric heterochromatin formation. In absence of this signal, PRC1 functions as the default repressive back-up mechanism. Parental epigenetic asymmetry, also observed along cleavage chromosomes, is resolved by the end of the 8-cell stage--concurrent with blastomere polarization--marking the end of the maternal-to-embryonic transition.
Similar articles
-
Polycomb group proteins Ezh2 and Rnf2 direct genomic contraction and imprinted repression in early mouse embryos.Dev Cell. 2008 Nov;15(5):668-79. doi: 10.1016/j.devcel.2008.08.015. Epub 2008 Oct 9. Dev Cell. 2008. PMID: 18848501
-
Cbx2 _targets PRC1 to constitutive heterochromatin in mouse zygotes in a parent-of-origin-dependent manner.Mol Cell. 2015 Apr 2;58(1):157-71. doi: 10.1016/j.molcel.2015.02.013. Epub 2015 Mar 19. Mol Cell. 2015. PMID: 25801166
-
Recruitment of PRC1 function at the initiation of X inactivation independent of PRC2 and silencing.EMBO J. 2006 Jul 12;25(13):3110-22. doi: 10.1038/sj.emboj.7601187. Epub 2006 Jun 8. EMBO J. 2006. PMID: 16763550 Free PMC article.
-
Biological functions of chromobox (CBX) proteins in stem cell self-renewal, lineage-commitment, cancer and development.Bone. 2021 Feb;143:115659. doi: 10.1016/j.bone.2020.115659. Epub 2020 Sep 24. Bone. 2021. PMID: 32979540 Review.
-
Switching between Epigenetic States at Pericentromeric Heterochromatin.Trends Genet. 2015 Nov;31(11):661-672. doi: 10.1016/j.tig.2015.09.003. Epub 2015 Sep 29. Trends Genet. 2015. PMID: 26431676 Review.
Cited by
-
Bmi1 facilitates primitive endoderm formation by stabilizing Gata6 during early mouse development.Genes Dev. 2012 Jul 1;26(13):1445-58. doi: 10.1101/gad.188193.112. Epub 2012 Jun 19. Genes Dev. 2012. PMID: 22713603 Free PMC article.
-
Insights to maternal regulation of the paternal genome in mammalian livestock embryos: A mini-review.Front Genet. 2022 Aug 19;13:909804. doi: 10.3389/fgene.2022.909804. eCollection 2022. Front Genet. 2022. PMID: 36061209 Free PMC article. Review.
-
Chromatin signatures and retrotransposon profiling in mouse embryos reveal regulation of LINE-1 by RNA.Nat Struct Mol Biol. 2013 Mar;20(3):332-8. doi: 10.1038/nsmb.2495. Epub 2013 Jan 27. Nat Struct Mol Biol. 2013. PMID: 23353788
-
Epigenetic programming and reprogramming during development.Nat Struct Mol Biol. 2013 Mar;20(3):282-9. doi: 10.1038/nsmb.2489. Epub 2013 Mar 5. Nat Struct Mol Biol. 2013. PMID: 23463313 Review.
-
PRC1 coordinates timing of sexual differentiation of female primordial germ cells.Nature. 2013 Mar 14;495(7440):236-40. doi: 10.1038/nature11918. Nature. 2013. PMID: 23486062
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases