doi: 10.1016/j.febslet.2008.05.010.
Epub 2008 May 20.
Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro
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- PMID: 18498772
- DOI: 10.1016/j.febslet.2008.05.010
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Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro
FEBS Lett.
.
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Abstract
MDCK cells expressing an inducible duodenal cytochrome b-green fluorescent protein (Dcytb-EGFP) fusion construct were used to investigate the function of Dcytb. The Dcytb-EGFP protein was _targeted correctly to the plasma membrane, and cells displayed increased ferric and cupric reductase activities, which were greatly reduced in the presence of doxycycline. The data suggests that Dcytb plays a physiological role in both iron and copper uptake, through divalent metal transporter 1 (DMT1) and copper transporter 1, respectively. In support of this hypothesis, we show that 59Fe uptake was significantly enhanced in Dcytb-EGFP expressing MDCK cells which endogenously express DMT1.
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