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. 2008 Mar 15;1(6):475-88.

Laser capture microdissection in the genomic and proteomic era: _targeting the genetic basis of cancer

Barbara Domazet  1 , Gregory T Maclennan, Antonio Lopez-Beltran, Rodolfo Montironi, Liang Cheng
Affiliations

Laser capture microdissection in the genomic and proteomic era: _targeting the genetic basis of cancer

Barbara Domazet et al. Int J Clin Exp Pathol. .

Abstract

The advent of new technologies has enabled deeper insight into processes at subcellular levels, which will ultimately improve diagnostic procedures and patient outcome. Thanks to cell enrichment methods, it is now possible to study cells in their native environment. This has greatly contributed to a rapid growth in several areas, such as gene expression analysis, proteomics, and metabolonomics. Laser capture microdissection (LCM) as a method of procuring subpopulations of cells under direct visual inspection is playing an important role in these areas. This review provides an overview of existing LCM technology and its downstream applications in genomics, proteomics, diagnostics and therapy.

Keywords: Laser capture microdissection; biomarker discovery; carcinogenesis; clonality; genomics; prognosis; proteomics.

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Figures

Figure 1
Figure 1
Components of the Artcurus laser capture microdissection system (PixCell II).
Figure 2
Figure 2
Tissue sources and applications of LCM
Figure 3
Figure 3
Example of microdissected cancer cells with LCM. Melanoma cells before (A) and after (B, C) microdissection.
See this image and copyright information in PMC

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References

    1. Suarez-Quian CA, Goldstein SR, Bonner RF. Laser capture microdissection: a new tool for the study of spermatogenesis. J Androl. 2000;21:601–608. - PubMed
    1. Espina V, Heiby M, Pierobon M, Liotta LA. Laser capture microdissection technology. Expert Rev Mol Diagn. 2007;7:647–657. - PubMed
    1. Espina V, Milia J, Wu G, Cowherd S, Liotta LA. Laser capture microdissection. Methods Mol Biol. 2006;319:213–229. - PubMed
    1. Simone NL, Paweletz CP, Charboneau L, Petricoin EF, 3rd, Liotta LA. Laser capture microdissection: beyond functional genomics to proteomics. Mol Diagn. 2000;5:301–507. - PubMed
    1. Craven RA, Banks RE. Use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis. Methods Enzymol. 2002;356:33–49. - PubMed

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