Review
doi: 10.1007/978-1-59745-527-5_3.
Modulating and _targeting meiotic double-strand breaks in Saccharomyces cerevisiae
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- PMID: 19799174
- DOI: 10.1007/978-1-59745-527-5_3
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Review
Modulating and _targeting meiotic double-strand breaks in Saccharomyces cerevisiae
Methods Mol Biol.
2009.
Abstract
Meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the evolutionary conserved Spo11 protein. Along the S. cerevisiae chromosomes, the DSB sites are not evenly distributed and the cleavage frequencies vary 10-100-fold from site to site. Herein are reviewed the methods used in budding yeast to modulate locally and globally the native DSB frequencies, including a powerful method to _target Spo11-dependent meiotic DSB in novel chromosomal regions. These methods serve to investigate the control and the mechanism of recombination initiation and modify the natural distribution of meiotic recombination.
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