Review
doi: 10.3760/cma.j.issn.1003-9406.2010.02.010.
[Zinc finger nucleases and their application]
[Article in
Chinese]
Affiliations
- PMID: 20376797
- DOI: 10.3760/cma.j.issn.1003-9406.2010.02.010
Item in Clipboard
Review
[Zinc finger nucleases and their application]
[Article in
Chinese]
Zhonghua Yi Xue Yi Chuan Xue Za Zhi.
2010 Apr.
Abstract
Zinc finger nuclease (ZFN), which is a chimeric fusion structure between a Cys2-His2 zinc-finger protein (ZFP) and the cleavage domain of Fok I endonuclease, can be used to introduce _targeted double-stranded breaks (DSBs). ZFN-mediated cleavage leads to mutations when double-stranded breaks are repaired by homologous recombination (HR) or nonhomologous end joining (NHEJ). In recent years, ZFNs are widely used in the fields of genetic research. In this review, the methodology and technical advantages of ZFNs were briefly discussed.
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