Genetic basis of Candida biofilm resistance due to drug-sequestering matrix glucan
- PMID: 20497051
- PMCID: PMC2880631
- DOI: 10.1086/651200
Genetic basis of Candida biofilm resistance due to drug-sequestering matrix glucan
Abstract
Medical devices provide an ecological niche for microbes to flourish as a biofilm community, protected from antimicrobials and host defenses. Biofilms formed by Candida albicans, the most common fungal pathogen, survive exposure to extraordinarily high drug concentrations. Here, we show that beta-glucan synthase Fks1p produces glucan, which is deposited in the biofilm matrix. The extracellular glucan is required for biofilm resistance and acts by sequestering antifungals, rendering cells resistant to their action. These findings provide the genetic basis for how biofilm matrix production governs drug resistance by impeding drug diffusion and also identify a useful biofilm drug _target.
Conflict of interest statement
Potential conflicts of interest: J. N. No conflict, H. S. No conflict, M.C. No conflict, D. A. No conflict
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References
-
- Gola S, Martin R, Walther A, Dunkler A, Wendland J. New modules for PCR-based gene _targeting in Candida albicans: rapid and efficient gene _targeting using 100 bp of flanking homology region. Yeast. 2003;20:1339–47. - PubMed
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