doi: 10.1038/nbt.1755.
Epub 2010 Dec 22.
A TALE nuclease architecture for efficient genome editing
Affiliations
- PMID: 21179091
- DOI: 10.1038/nbt.1755
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A TALE nuclease architecture for efficient genome editing
Nat Biotechnol.
2011 Feb.
Abstract
Nucleases that cleave unique genomic sequences in living cells can be used for _targeted gene editing and mutagenesis. Here we develop a strategy for generating such reagents based on transcription activator-like effector (TALE) proteins from Xanthomonas. We identify TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and use these nucleases to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%. We further show that designed TALEs can regulate endogenous mammalian genes. These studies demonstrate the effective application of designed TALE transcription factors and nucleases for the _targeted regulation and modification of endogenous genes.
Comment in
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TALEs of genome _targeting.Nat Biotechnol. 2011 Feb;29(2):135-6. doi: 10.1038/nbt.1767. Nat Biotechnol. 2011. PMID: 21301438 No abstract available.
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TALEs for the masses.Nat Methods. 2011 Mar;8(3):197. doi: 10.1038/nmeth0311-197. Nat Methods. 2011. PMID: 21473016
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Knockout rats generated by embryo microinjection of TALENs.Nat Biotechnol. 2011 Aug 5;29(8):695-6. doi: 10.1038/nbt.1940. Nat Biotechnol. 2011. PMID: 21822240 No abstract available.
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_targeted gene disruption in somatic zebrafish cells using engineered TALENs.Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. Nat Biotechnol. 2011. PMID: 21822241 Free PMC article. No abstract available.
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Heritable gene _targeting in zebrafish using customized TALENs.Nat Biotechnol. 2011 Aug 5;29(8):699-700. doi: 10.1038/nbt.1939. Nat Biotechnol. 2011. PMID: 21822242 No abstract available.
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