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. 2011 Mar;14(3):219-26.
doi: 10.3779/j.issn.1009-3419.2011.03.07.

[The expression and clinical significance of HIF-1α, COX-2 and E-cadherin in patients with lung adenocarcinoma]

[Article in Chinese]
Songtao Gu  1 , Jianwen Qin
Affiliations

[The expression and clinical significance of HIF-1α, COX-2 and E-cadherin in patients with lung adenocarcinoma]

[Article in Chinese]
Songtao Gu et al. Zhongguo Fei Ai Za Zhi. 2011 Mar.

Abstract
in English, Chinese

Background and objective: The incidence of lung adenocarcinoma increases rapidly, and hypoxia-inducible factor-1α (HIF-1α), cyclooxygenase-2 (COX-2), E-cadherin play an important role in the proliferation and differentiation of cancer cell. The aim of this study is to investigate the clinical significance of the expression of HIF-1α, COX-2, E-cadherin in patients with lung adenocarcinoma and the internal relationship among them.

Methods: The expression levels of HIF-1α, COX-2 and E-cadherin were determined in 10 cases of normal lung issue and in 45 cases of issue of lung adenocarcinoma by immunohistochemical method.

Results: The positive expression rate of HIF-1α and COX-2 was 60% (27/45) and 40% (18/45) respectively in 45 cases of lung adenocarcinoma, was null in 10 cases of normal lung issue. The positive expression rate of E-cadherin was 48.9% (22/45) in 45 cases of lung adenocarcinoma, was 100% in 10 cases of normal lung issue. The expression of HIF-1α was positively correlated with the size of the cancer (P < 0.05), but not with age, smoking, lymphatic metastasis, differentiated degree and surgical-pathologic staging (P > 0.05). The expression of COX-2 was positively correlated with the size of the cancer, lymphatic metastasis, surgical-pathologic and expression of HIF-1α (P < 0.05), but not with age, smoking and differentiated degree (P > 0.05). The expression of E-cadherin was positively correlated with differentiated degree and lymphatic metastasis (P < 0.05), but not with age, smoking, the size of the cancer, surgical-pathologic and expression of HIF-1α (P > 0.05).

Conclusions: The overexpression of HIF-1α and COX-2 and the downregulation of E-cadherin is found in lung adenocarcinoma. The overexpression of HIF-1α may induce the overexpression of COX-2, and is non-correlated with expression of E-cadherin.

背景与目的: 肺腺癌发病率不断升高，而低氧诱导因子-1α（hypoxia-inducible factor-1α, HIF-1α）、环氧合酶-2（cyclooxygenase-2, COX-2）、上皮型钙粘附分子（E-cadherin）均在肿瘤细胞的分化、增殖过程中起到重要作用。本研究旨在探讨HIF-1α和COX-2、E-cadherin在肺腺癌的表达水平与患者临床病理特征之间的关系及其三者之间的内在联系。

方法: 收集10例非肿瘤患者手术切除的正常肺组织及45例肺腺癌患者手术切除标本，应用免疫组织化学方法检测HIF-1α、COX-2、E-cadherin的表达情况。

结果: 45例肺腺癌组织中，HIF-1α和COX-2的表达阳性率分别为60%（27/45）和40%（18/45），10例正常肺组织均未见表达。45例肺腺癌组织中E-cadherin的表达阳性率为48.9%（22/45），10例正常肺组织均见阳性表达。HIF-1α表达水平与原发肿瘤大小有密切关系（P < 0.05），但与患者年龄、吸烟与否、淋巴结转移、分化程度、术后分期无明显关系（P>0.05）。COX-2表达水平与原发肿瘤大小、淋巴结转移、术后分期、HIF-1α表达水平有密切关系（P < 0.05），但与患者年龄、吸烟与否、分化程度无明显关系（P>0.05）。E-cadherin表达水平与分化程度、淋巴结转移有密切关系（P < 0.05），但与患者年龄、吸烟与否、肿瘤最大直径、术后分期、HIF-1α的表达无明显关系（P>0.05）。

结论: 肺腺癌组织中HIF-1α、COX-2表达增高，E-cadherin表达降低；COX-2的表达水平升高可能与HIF-1α的高表达有关，E-cadherin的表达水平与HIF-1α未发现有明显相关性。

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Figures

1
1
HIF-1α蛋白的表达。A：正常支气管粘膜上皮细胞HIF-1α蛋白表达阴性（SP, ×100）；B：肺腺癌切片相邻正常支气管粘膜上皮细胞HIF-1α阳性表达（SP, ×100）；C, D：HIF-1α在肺腺癌的阳性表达，腺癌细胞胞浆和胞核可见棕黄色颗粒（SP, ×400） Expression of HIF-1α Protein. A: negative expression in normal bronchial epithelium cells (SP, ×100); B: positive expression in normal bronchial epithelium cells next to the lung adenocarcinoma (SP, ×100); C, D: positive expression in the lung adenocarcinoma cells of which there are many brown particles in the cytoplasm and cytoblast (SP, ×400)
2
2
COX-2蛋白的表达。A：COX-2蛋白在肺腺癌的阳性表达，胞浆棕黄色的颗粒（SP，×100）；B：COX-2在正常支气管粘膜上皮细胞的阴性表达（SP，×100）；C, D：COX-2在肺腺癌阳性表达（SP，×400） Expression of COX-2 protein. A: positive expression in the lung adenocarcionoma of which there are many brown particles in the cytoplasm (SP, ×100); B: negative expression in normal bronchial epithelium cells (SP, ×100); C, D: positive expression in the lung adenocarcionma (SP, ×400)
3
3
E-cadherin蛋白的表达。A：E-cadherin在正常支气管粘膜上皮细胞的正常表达，胞膜可见棕色颗粒沉着（SP，×100）；B：E-cadherin在正常支气管粘膜上皮细胞的正常表达（SP，×400）；C：E-cadherin蛋白在高分化腺癌的表达（SP，×100）；D：E-cadherin蛋白在中分化腺癌的表达（SP，×100）；E：E-cadherin蛋白在低分化腺癌的表达（SP，×100）；F：E-cadherin蛋白在肺腺癌相邻正常支气管粘膜上皮的表达（SP，×100） Expression of E-cadherin protein. A: positive expression in normal bronchial epithelium cells of which there are many brown par ticles in the cell membrance (SP, ×100); B : positive staining in normal bronchial epithelium cells (SP, ×40 0); C: expression in the lung welldif ferentiated adenocarcinoma (SP, ×100); D: expression in the lung moderate-differentiated adenocarcinoma (SP, ×100); E: expression in the lung poor-differentiated adenocarcinoma (SP, ×100); F: expression in normal bronchial epithelium cells next to the lung adenocarcinoma (SP, ×100)
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