Postinduction turnoff of beta-interferon gene expression
- PMID: 2157136
- PMCID: PMC362234
- DOI: 10.1128/mcb.10.4.1329-1337.1990
Postinduction turnoff of beta-interferon gene expression
Abstract
Viral induction of the human beta-interferon (IFN-beta) gene leads to a transient accumulation of high levels of IFN-beta mRNA. Previous studies have shown that the increase in IFN-beta mRNA levels after induction is due to an increase in the rate of IFN-beta gene transcription. In this paper, we show that the rapid postinduction decrease in the level of IFN-beta mRNA is due to a combination of transcriptional repression and rapid turnover of the mRNA. This transcriptional repression can be blocked with cycloheximide, suggesting that the synthesis of a virus-inducible repressor is necessary for the postinduction turnoff of the IFN-beta gene. Analysis of the sequence requirements for IFN-beta mRNA instability revealed two regions capable of destabilizing a heterologous mRNA. One destabilizer is an AU-rich sequence in the 3' untranslated region, and the other is located 5' to the translation stop codon.
Similar articles
-
Postinduction repression of the beta-interferon gene is mediated through two positive regulatory domains.Proc Natl Acad Sci U S A. 1990 Oct;87(20):7799-803. doi: 10.1073/pnas.87.20.7799. Proc Natl Acad Sci U S A. 1990. PMID: 2172963 Free PMC article.
-
Priming affects the activity of a specific region of the promoter of the human beta interferon gene.Mol Cell Biol. 1990 Feb;10(2):854-8. doi: 10.1128/mcb.10.2.854-858.1990. Mol Cell Biol. 1990. PMID: 2153928 Free PMC article.
-
Virus infection and interferon can activate gene expression through a single synthetic element, but endogenous genes show distinct regulation.J Biol Chem. 1989 Oct 5;264(28):16658-66. J Biol Chem. 1989. PMID: 2550451
-
Induction of human interferon gene expression is associated with a nuclear factor that interacts with the NF-kappa B site of the human immunodeficiency virus enhancer.J Virol. 1989 Jun;63(6):2557-66. doi: 10.1128/JVI.63.6.2557-2566.1989. J Virol. 1989. PMID: 2542571 Free PMC article.
-
Transcriptional repression of type I IFN genes.Biochimie. 1998 Aug-Sep;80(8-9):689-701. doi: 10.1016/s0300-9084(99)80022-4. Biochimie. 1998. PMID: 9865491 Review.
Cited by
-
Association of the interferon-β gene with pericentromeric heterochromatin is dynamically regulated during virus infection through a YY1-dependent mechanism.Nucleic Acids Res. 2012 May;40(10):4396-411. doi: 10.1093/nar/gks050. Epub 2012 Jan 28. Nucleic Acids Res. 2012. PMID: 22287632 Free PMC article.
-
XBP-1 couples endoplasmic reticulum stress to augmented IFN-beta induction via a cis-acting enhancer in macrophages.J Immunol. 2010 Aug 15;185(4):2324-30. doi: 10.4049/jimmunol.0903052. Epub 2010 Jul 21. J Immunol. 2010. PMID: 20660350 Free PMC article.
-
The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity.J Biol Chem. 2020 Jan 10;295(2):444-457. doi: 10.1074/jbc.RA119.010511. Epub 2019 Nov 25. J Biol Chem. 2020. PMID: 31767682 Free PMC article.
-
Identification of distal silencing elements in the murine interferon-A11 gene promoter.Biochem J. 1996 Aug 1;317 ( Pt 3)(Pt 3):697-706. doi: 10.1042/bj3170697. Biochem J. 1996. PMID: 8760352 Free PMC article.
-
Translational regulation of human beta interferon mRNA: association of the 3' AU-rich sequence with the poly(A) tail reduces translation efficiency in vitro.Mol Cell Biol. 1993 Jun;13(6):3487-93. doi: 10.1128/mcb.13.6.3487-3493.1993. Mol Cell Biol. 1993. PMID: 7684500 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
