Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2009 Dec 29;53(4):e27.
doi: 10.4081/ejh.2009.e27.

Identification of the novel localization of tenascinX in the monkey choroid plexus and comparison with the mouse

K Imura  1 , I Sato
Affiliations
Comparative Study

Identification of the novel localization of tenascinX in the monkey choroid plexus and comparison with the mouse

K Imura et al. Eur J Histochem. .

Abstract

Tenascin-X (Tn-X) belongs to the tenascin family of glycoproteins and has been reported to be significantly associated with schizophrenia in a single nucleotide polymorphism analysis in humans. This finding indicates an important role of Tn-X in the central nervous system (CNS). However, details of Tn-X localization are not clear in the primate CNS. Using immunohistochemical techniques, we found novel localizations of Tn-X in the interstitial connective tissue and around blood vessels in the choroid plexus (CP) in macaque monkeys. To verify the reliability of Tn-X localization, we compared the Tn-X localization with the tenascin-C (Tn-C) localization in corresponding regions using neighbouring sections. Localization of Tn-C was not observed in CP. This result indicated consistently restricted localization of Tn-X in CP. Comparative investigations using mouse tissues showed equivalent results. Our observations provide possible insight into specific roles of Tn-X in CP for mammalian CNS function.

Keywords: Ehlers-Danlos syndrome; choroid plexus; monkey; mouse; schizophrenia.; tenascin-X.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Immunohistochemistry (IHC) of tenascin-X (Tn-X) and tenascin-C (Tn-C) in monkey choroid plexus (CP) in fourth ventricle. (A) Low magnification of IHC of Tn-X in the lateral recess of fourth ventricle. Two boxed regions are re-photographed at higher magnification in B and D. (B, D) Tn-X positive fibrous structures are clearly observed within connective tissue of CP (arrows). (C, E) IHC of the Tn-C in neighboring sections to the B and D. Tn-C positive fibrous structures are not detected within the connective tissue of CP. CC: cerebellar cortex, EC: epithelial cell, MO: medulla oblongata. Scale bars=500 µm in A, 50 µm in B–E.
Figure 2
Figure 2
Tenascin-X (Tn-X) positive vascular and leptomenigeal structures in monkey choroid plexus (CP), cerebellar cortex (CC), and medulla oblongata (MO). (A, B) Tn-X positive fibrous structures are clearly observed around blood vessels (asterisks) and within the connective tissue of CP in lateral ventricle (arrowheads). (C) Leptomeninges on the CC is strongly Tn-X positive (arrowheads). An arrow indicates Tn-X positive blood vessel. (D) Immunohistochemistry of tenascin -C in a neighboring section to the C. Tn-C positive leptomeninges is not observed in the CC (arrowheads). An arrow indicates Tn-C positive blood vessel, which is corresponding to the Tn-X positive blood vessel in C (arrow). E: Tn-X positive blood vessels are also observed in MO (arrows). EC: epithelial cell. Scale bars=50 µm.
Figure 3
Figure 3
Immunohistochemistry (IHC) of the tenascin-X (Tn-X) and tenascin-C (Tn-C) in mouse choroid plexus (CP) in fourth ventricle. (A) Low magnification of IHC of Tn-X in a coronal section at fourth ventricle level. One boxed region is re-photographed at higher magnification in B. (B, D) Tn-X positive fibrous structures are clearly observed within connective tissue of CP. Three arrowheads in B indicate Tn-X positive leptomeninges on PFl (paraflocculus). The panel D is photographed from an another section. (C, E) IHC of the Tn C in neighboring sections to the B and D. Tn C positive fibrous structures are not detected within the connective tissue of CP and leptomeninges. 4V: fourth ventricle, CC: cerebellar cortex, EC: epithelial cell, LR4V: lateral recess of 4V, MO: medulla oblongata. Scale bars=500 µm in A, 50 µm in B–E.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Aase K, Lymboussaki A, Kaipainen A, Olofsson B, Alitalo K, Eriksson U. Localization of VEGF-B in the mouse embryo suggests a paracrine role of the growth factor in the developing vasculature. Dev Dyn. 1999;215:1–25. - PubMed
    1. Adams JC, Watt FM. Regulation of development and differentiation by the extracellular matrix. Development. 1993;117:1183–98. - PubMed
    1. Ballard VLT, Sharma A, Duignan I, Holm JM, Chin A, Choi R, Hajjar KA, Wong SC, Edelberge JM. Vascular tenascin-C regulates cardiac endothelial phenotype and neovascularization. FASEB J. 2006;20:717–9. - PubMed
    1. Brown PD, Davies SL, Speake T, Millar ID. Molecular mechanisms of cerebrospinal fluid production. Neuroscience. 2004;129:957–70. - PMC - PubMed
    1. Borlongan CV, Skinner SJM, Geaney M, Vasconcellos AV, Elliott RB, Emerich DF. Intracerebral transplantation of porcine choroid plexus provides structural and functional neuroprotection in a rodent model of stroke. Stroke. 2004;35:2206–10. - PubMed

Publication types

  NODES
twitter 2
Verify 1