Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 May;124(8):1549-60.
doi: 10.1007/s00122-012-1809-7. Epub 2012 Feb 18.

High-density mapping and marker development for the powdery mildew resistance gene PmAS846 derived from wild emmer wheat (Triticum turgidum var. dicoccoides)

Fei Xue  1 , Wanquan Ji, Changyou Wang, Hong Zhang, Baoju Yang
Affiliations

High-density mapping and marker development for the powdery mildew resistance gene PmAS846 derived from wild emmer wheat (Triticum turgidum var. dicoccoides)

Fei Xue et al. Theor Appl Genet. 2012 May.

Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is an important foliar disease of wheat worldwide. The dominant powdery mildew resistance gene PmAS846 was transferred to the hexaploid wheat lines N9134 and N9738 from wild emmer wheat (Triticum dicoccoides) in 1995, and it is still one of the most effective resistance genes in China. A high resolution genetic map for PmAS846 locus was constructed using two F(2) populations and corresponding F(2:3) families developed from the crosses of N9134/Shaanyou 225 and N9738/Huixianhong. Synteny between wheat and Brachypodium distachyon and rice was used to develop closely linked molecular markers to reduce the genetic interval around PmAS846. Twenty-six expressed sequence tag-derived markers were mapped to the PmAS846 locus. Five markers co-segregated with PmAS846 in the F(2) population of N9134/Shaanyou 225. PmAS846 was physically located to wheat chromosome 5BL bin 0.75-0.76 within a gene-rich region. The markers order is conserved between wheat and Brachypodium distachyon, but rearrangements are present in rice. Two markers, BJ261635 and CJ840011 flanked PmAS846 and narrowed PmAS846 to a region that is collinear with 197 and 112 kb genomic regions on Brachypodium chromosome 4 and rice chromosome 9, respectively. The genes located on the corresponding homologous regions in Brachypodium, rice and barley could be considered for further marker saturation and identification of potential candidate genes for PmAS846. The markers co-segregating with PmAS846 provide a potential _target site for positional cloning of PmAS846, and can be used for marker-assisted selection of this gene.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. BMC Bioinformatics. 2009 Oct 13;10:331 - PubMed
    1. Mol Ecol. 2000 Nov;9(11):1699-710 - PubMed
    1. Phytopathology. 1998 Feb;88(2):144-7 - PubMed
    1. Genetics. 2003 Jun;164(2):655-64 - PubMed
    1. Proc Natl Acad Sci U S A. 2011 May 10;108(19):7727-32 - PubMed

Publication types

Substances

LinkOut - more resources

  NODES
twitter 2