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. 2012 Jun;19(6):914-8.
doi: 10.1128/CVI.05630-11. Epub 2012 Apr 25.

Comparative analysis of the humoral immune response to Moraxella catarrhalis and Streptococcus pneumoniae surface antigens in children suffering from recurrent acute otitis media and chronic otitis media with effusion

Suzanne J C Verhaegh  1 , Kim Stol, Corné P de Vogel, Kristian Riesbeck, Eric R Lafontaine, Timothy F Murphy, Alex van Belkum, Peter W M Hermans, John P Hays
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Comparative analysis of the humoral immune response to Moraxella catarrhalis and Streptococcus pneumoniae surface antigens in children suffering from recurrent acute otitis media and chronic otitis media with effusion

Suzanne J C Verhaegh et al. Clin Vaccine Immunol. 2012 Jun.

Abstract

A prospective clinical cohort study was established to investigate the humoral immune response in middle ear fluids (MEF) and serum against bacterial surface proteins in children suffering from recurrent acute otitis media (rAOM) and chronic otitis media with effusion (COME), using Luminex xMAP technology. The association between the humoral immune response and the presence of Moraxella catarrhalis and Streptococcus pneumoniae in the nasopharynx and middle ear was also studied. The levels of antigen-specific IgG, IgA, and IgM showed extensive interindividual variation. No significant differences in anti-M. catarrhalis and anti-S. pneumoniae serum and MEF median fluorescence intensity (MFI) values (anti-M. catarrhalis and antipneumococcal IgG levels) were observed between the rAOM or COME groups for all antigens tested. No significant differences were observed for M. catarrhalis and S. pneumoniae colonization and serum IgG levels against the Moraxella and pneumococcal antigens. Similar to the antibody response in serum, no significant differences in IgG, IgA, and IgM levels in MEF were observed for all M. catarrhalis and S. pneumoniae antigens between OM M. catarrhalis- or S. pneumoniae-positive and OM M. catarrhalis- or S. pneumonia-negative children suffering from either rAOM or COME. Finally, results indicated a strong correlation between antigen-specific serum and MEF IgG levels. We observed no significant in vivo expressed anti-M. catarrhalis or anti-S. pneumoniae humoral immune responses using a range of putative vaccine candidate proteins. Other factors, such as Eustachian tube dysfunction, viral load, and genetic and environmental factors, may play a more important role in the pathogenesis of OM and in particular in the development of rAOM or COME.

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Figures

Fig 1
Fig 1
Correlation between IgG levels in serum and MEF. Mean IgG levels in serum and MEF were used to calculate the correlation statistics for each respective M. catarrhalis (A) or S. pneumoniae (B) protein (Spearman's correlation coefficient, R2 = 0.97 and 0.89).
Fig 2
Fig 2
Example of the results obtained; specifically, the figure shows a comparison of antipneumococcal IgA-1 protease IgA levels in COME-positive children in the absence and presence of S. pneumoniae in the middle ear.
See this image and copyright information in PMC

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