A novel peptide which stimulates adenylate cyclase: molecular cloning and characterization of the ovine and human cDNAs
- PMID: 2302217
- DOI: 10.1016/0006-291x(90)91914-e
A novel peptide which stimulates adenylate cyclase: molecular cloning and characterization of the ovine and human cDNAs
Abstract
A novel neuropeptide which remarkably stimulates adenylate cyclase in rat anterior pituitary cell cultures has been recently isolated from ovine hypothalami by A. Arimura and his collaborators (Biochem.Biophys.Res.Commun.164, 567-574(1989)). This peptide was designated as PACAP38(Pituitary Adenylate Cyclase Activating Polypeptide with 38 residues). In an attempt to investigate physiological implications of PACAP38, we have succeeded in cloning the cDNAs encoding the precursor of PACAP38 from ovine hypothalamus and human testis. An ovine cDNA encodes a protein of 176 amino acids in which PACAP38 is proceeded by a putative signal peptide and a "pro"-region (107 amino acids), and followed by a Gly-Arg-Arg sequence for proteolytic processing and amidation. Deduced amino-acid sequence of human PACAP38 was completely identical to that of the ovine isolated peptide. Cloning of PACAP38 cDNAs confirms the expression of the corresponding mRNAs and the presence of this neuropeptide in ovine hypothalamus and also in human testis.
Similar articles
-
Isolation of a neuropeptide corresponding to the N-terminal 27 residues of the pituitary adenylate cyclase activating polypeptide with 38 residues (PACAP38).Biochem Biophys Res Commun. 1990 Jul 31;170(2):643-8. doi: 10.1016/0006-291x(90)92140-u. Biochem Biophys Res Commun. 1990. PMID: 2383262
-
Molecular cloning and characterization of cDNA for the precursor of rat pituitary adenylate cyclase activating polypeptide (PACAP).Biochem Biophys Res Commun. 1990 Dec 31;173(3):1271-9. doi: 10.1016/s0006-291x(05)80924-6. Biochem Biophys Res Commun. 1990. PMID: 2268329
-
Primary structure and characterization of the precursor to human pituitary adenylate cyclase activating polypeptide.DNA Cell Biol. 1992 Jan-Feb;11(1):21-30. doi: 10.1089/dna.1992.11.21. DNA Cell Biol. 1992. PMID: 1739432
-
[Pituitary adenylate cyclase-activating polypeptide].Ann Endocrinol (Paris). 1998 Dec;59(5):364-405. Ann Endocrinol (Paris). 1998. PMID: 9949891 Review. French.
-
Sensitization: a mechanism for melatonin action in the pars tuberalis.J Neuroendocrinol. 2003 Apr;15(4):415-21. doi: 10.1046/j.1365-2826.2003.00988.x. J Neuroendocrinol. 2003. PMID: 12622843 Review.
Cited by
-
PACAP orchestration of stress-related responses in neural circuits.Peptides. 2021 Aug;142:170554. doi: 10.1016/j.peptides.2021.170554. Epub 2021 Apr 15. Peptides. 2021. PMID: 33865930 Free PMC article. Review.
-
PAC1 Receptor Internalization and Endosomal MEK/ERK Activation Is Essential for PACAP-Mediated Neuronal Excitability.J Mol Neurosci. 2021 Aug;71(8):1536-1542. doi: 10.1007/s12031-021-01821-x. Epub 2021 Mar 6. J Mol Neurosci. 2021. PMID: 33675454 Free PMC article. Review.
-
Transcriptomics and proteomics analyses of the PACAP38 influenced ischemic brain in permanent middle cerebral artery occlusion model mice.J Neuroinflammation. 2012 Nov 23;9:256. doi: 10.1186/1742-2094-9-256. J Neuroinflammation. 2012. PMID: 23176072 Free PMC article.
-
PACAP38 and PAC1 receptor blockade: a new _target for headache?J Headache Pain. 2018 Aug 7;19(1):64. doi: 10.1186/s10194-018-0893-8. J Headache Pain. 2018. PMID: 30088106 Free PMC article. Review.
-
Roles for pituitary adenylate cyclase-activating peptide (PACAP) expression and signaling in the bed nucleus of the stria terminalis (BNST) in mediating the behavioral consequences of chronic stress.J Mol Neurosci. 2010 Nov;42(3):327-40. doi: 10.1007/s12031-010-9364-7. Epub 2010 Apr 20. J Mol Neurosci. 2010. PMID: 20405238 Free PMC article. Review.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
