Genetically encoded Ca(2+) indicators: properties and evaluation
- PMID: 23352808
- DOI: 10.1016/j.bbamcr.2013.01.011
Genetically encoded Ca(2+) indicators: properties and evaluation
Abstract
Genetically encoded calcium ion (Ca(2+)) indicators have become very useful and widely used tools for Ca(2+) imaging, not only in cellular models, but also in living organisms. However, the in vivo and in situ characterization of these indicators is tedious and time consuming, and it does not provide information regarding the suitability of an indicator for particular experimental environments. Thus, initial in vitro evaluation of these tools is typically performed to determine their properties. In this review, we examined the properties of dynamic range, affinity, selectivity, and kinetics for Ca(2+) indicators. Commonly used strategies for evaluating these properties are presented. This article is part of a Special Issue entitled: 12th European Symposium on Calcium.
Copyright © 2013 Elsevier B.V. All rights reserved.
Similar articles
-
Genetically Encoded Fluorescent Indicators for Organellar Calcium Imaging.Biophys J. 2016 Sep 20;111(6):1119-1131. doi: 10.1016/j.bpj.2016.04.054. Epub 2016 Jul 29. Biophys J. 2016. PMID: 27477268 Free PMC article. Review.
-
Recent progress in the development of genetically encoded Ca2+ indicators.J Med Invest. 2015;62(1-2):24-8. doi: 10.2152/jmi.62.24. J Med Invest. 2015. PMID: 25817279 Review.
-
Deleterious effects of calcium indicators within cells; an inconvenient truth.Cell Calcium. 2018 Jul;73:82-87. doi: 10.1016/j.ceca.2018.04.005. Epub 2018 Apr 15. Cell Calcium. 2018. PMID: 29689523 Review.
-
Evaluation of multi-color genetically encoded Ca2+ indicators in filamentous fungi.Fungal Genet Biol. 2021 Apr;149:103540. doi: 10.1016/j.fgb.2021.103540. Epub 2021 Feb 16. Fungal Genet Biol. 2021. PMID: 33607281
-
Optical probing of neuronal circuit dynamics: genetically encoded versus classical fluorescent sensors.Trends Neurosci. 2006 Mar;29(3):160-6. doi: 10.1016/j.tins.2006.01.004. Epub 2006 Jan 27. Trends Neurosci. 2006. PMID: 16443289 Review.
Cited by
-
A protocol for imaging calcium and chloride in C. elegans glia upon touch stimulation.STAR Protoc. 2022 Apr 13;3(2):101282. doi: 10.1016/j.xpro.2022.101282. eCollection 2022 Jun 17. STAR Protoc. 2022. PMID: 35463465 Free PMC article.
-
Sensing Senses: Optical Biosensors to Study Gustation.Sensors (Basel). 2020 Mar 25;20(7):1811. doi: 10.3390/s20071811. Sensors (Basel). 2020. PMID: 32218129 Free PMC article. Review.
-
Updated Toolbox for Assessing Neuronal Network Reconstruction after Cell Therapy.Bioengineering (Basel). 2024 May 14;11(5):487. doi: 10.3390/bioengineering11050487. Bioengineering (Basel). 2024. PMID: 38790353 Free PMC article. Review.
-
High-throughput-compatible assays using a genetically-encoded calcium indicator.Sci Rep. 2019 Sep 3;9(1):12692. doi: 10.1038/s41598-019-49070-8. Sci Rep. 2019. PMID: 31481721 Free PMC article.
-
Bioluminescent Sensors for Ca++ Flux Imaging and the Introduction of a New Intensity-Based Ca++ Sensor.Front Bioeng Biotechnol. 2021 Oct 27;9:773353. doi: 10.3389/fbioe.2021.773353. eCollection 2021. Front Bioeng Biotechnol. 2021. PMID: 34778237 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
