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Review
. 2013 Mar 1;5(3):a013169.
doi: 10.1101/cshperspect.a013169.

Endoplasmic reticulum stress sensing in the unfolded protein response

Brooke M Gardner  1 , David Pincus, Katja Gotthardt, Ciara M Gallagher, Peter Walter
Affiliations
Review

Endoplasmic reticulum stress sensing in the unfolded protein response

Brooke M Gardner et al. Cold Spring Harb Perspect Biol. .

Abstract

Secretory and transmembrane proteins enter the endoplasmic reticulum (ER) as unfolded proteins and exit as either folded proteins in transit to their _target organelles or as misfolded proteins _targeted for degradation. The unfolded protein response (UPR) maintains the protein-folding homeostasis within the ER, ensuring that the protein-folding capacity of the ER meets the load of client proteins. Activation of the UPR depends on three ER stress sensor proteins, Ire1, PERK, and ATF6. Although the consequences of activation are well understood, how these sensors detect ER stress remains unclear. Recent evidence suggests that yeast Ire1 directly binds to unfolded proteins, which induces its oligomerization and activation. BiP dissociation from Ire1 regulates this oligomeric equilibrium, ultimately modulating Ire1's sensitivity and duration of activation. The mechanistic principles of ER stress sensing are the focus of this review.

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Figures

Figure 1.
Figure 1.
In metazoans, three parallel signaling pathways comprise the UPR. ER-resident transmembrane sensor proteins, Ire1, PERK, and ATF6, activate signaling in each UPR branch. Upon activation, each sensor elicits unique downstream responses. Ire1’s cytosolic RNase domain cleaves an intron out of an mRNA, leading to the production of a potent transcriptional activator that induces genes to increase the folding capacity of the ER (XBP1 in metazoans, Hac1 in budding yeast). The active RNase also cleaves ER-localized messages, leading to their degradation, to reduce the load of unfolded proteins entering the ER. Active ATF6 translocates from the ER to the Golgi where it is proteolytically processed to release its amino-terminal transcriptional activator domain that induces genes to increase the folding capacity in the ER. PERK’s cytosolic kinase domain phosphorylates the translation initiation factor eIF2α, thereby inhibiting global translation and reducing the load of newly synthesized proteins entering the ER. Although generally inhibiting translation, eIF2α phosphorylation allows messages with inhibitory leader sequences to be preferentially translated. One of these messages encodes ATF4, a transcriptional activator that induces a cascade that ultimately produces proapoptotic factors.
Figure 2.
Figure 2.
Unfolded proteins are the switch that activates Ire1, while BiP binding regulates the sensitivity and duration of the response. (A) Ire1 is in equilibrium between monomeric and oligomeric states. Binding to BiP stabilizes the monomeric state by providing a sink to buffer the amount of free Ire1. Binding to unfolded proteins stabilizes the oligomeric state by overcoming the activation barrier for Ire1 oligomerization. (B) BiP dissociation from Ire1 is not sufficient to activate Ire1 (panel B is from Pincus et al. 2010; reprinted, with permission, from the authors). (Upper panel) Quantification of coimmunoprecipitation experiments showing that BiP dissociates from wild-type Ire1 in the presence of ER stress. A mutant of Ire1 lacking the ER juxtamembrane segment (Ire1bipless) binds to BiP in the absence of stress only to the degree that wild-type Ire1 binds to BiP in the presence of stress. In ER stress conditions, Ire1bipless shows no change in its association with BiP. (Lower panel) Northern blot measuring splicing of HAC1 mRNA showing that, despite its lack of ER-stress-dependent BiP dissociation, the RNase of Ire1bipless is not constitutively active and remains ER-stress inducible. (C) Peptide binding triggers Ire1-LD oligomerization in vitro (panel C is from Gardner and Walter 2011; reprinted, with permission, from the authors). Velocity sedimentation analysis of recombinant Ire1-LD in the presence and absence of a peptide proxy for an unfolded protein shows that Ire1-LD redistributes into large oligomeric assemblies in the presence of peptide. (D) Mathematical model simulation predicts that Ire1bipless would display delayed deactivation kinetics compared to wild-type Ire1 once ER stress is removed (panel D is from Pincus et al. 2010; reprinted, with permission, from the author). (E) Experimental verification that Ire1bipless deactivates less efficiently than wild-type Ire1 once ER stress is removed (panel E is from Pincus et al. 2010; reprinted, with permission, from the authors). FRET measurements between Ire1 molecules reveals that deoligomerization is impaired in the Ire1bipless mutant.
Figure 3.
Figure 3.
Crystal structure of yeast and human Ire1-LD indicate how Ire1 oligomerizes. (A) The S. cerevisiae Ire1-LD dimer formed at Interface 1, creating a putative ligand-binding groove (PDB: 2BE1). Each monomer is colored red to blue from amino to carboxyl terminus. Compare to Figure 4A for a surface representation. (B) The H. sapiens Ire1-LD dimer formed at Interface 1 has similar architecture to yeast Ire1-LD, though the α-helical walls narrow the putative ligand binding groove (PDB: 2HZ6). Each monomer is colored red to blue from amino to carboxyl terminus. Compare to Figure 4B for a surface representation. (C) Two groove-forming dimers of yeast Ire1-LD oligomerize through Interface 2. An α-helical turn region (arrowhead) interacts with a β-sheet (arrow) to mediate this interaction. Residues that have been mutated and shown to impair UPR activation are colored red. (D) Two groove-forming dimers of human Ire1-LD interact through a novel Interface 2. The interaction between the β-sheet (arrow) and the α-helical turn region, unstructured here (arrowhead), that formed Interface 2 in the yeast Ire1-LD is now interrupted by the long helix αB. Figures were made using PyMOL (PyMOL Molecular Graphics System).
Figure 4.
Figure 4.
Crystal structures of Ire1-LD reveal a putative ligand-binding groove similar to those of MHC and DnaK. Surface models of the structures of (A) yeast Ire1-LD, (B) human Ire1-LD, (C) MHCII peptide binding domain crystallized with a peptide ligand (magenta; PDB: 1DLH), and (D) DnaK substrate binding domain crystallized with a peptide ligand (magenta; PDB: 1DKX). All structures are colored white to blue by depth looking down on the proposed or proven ligand-binding groove. Capped side views show a cross section of the ligand-binding groove to illustrate the pockets available to bind anchor residues.
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