Circular intronic long noncoding RNAs
- PMID: 24035497
- DOI: 10.1016/j.molcel.2013.08.017
Circular intronic long noncoding RNAs
Abstract
We describe the identification and characterization of circular intronic long noncoding RNAs in human cells, which accumulate owing to a failure in debranching. The formation of such circular intronic RNAs (ciRNAs) can be recapitulated using expression vectors, and their processing depends on a consensus motif containing a 7 nt GU-rich element near the 5' splice site and an 11 nt C-rich element close to the branchpoint site. In addition, we show that ciRNAs are abundant in the nucleus and have little enrichment for microRNA _target sites. Importantly, knockdown of ciRNAs led to the reduced expression of their parent genes. One abundant such RNA, ci-ankrd52, largely accumulates to its sites of transcription, associates with elongation Pol II machinery, and acts as a positive regulator of Pol II transcription. This study thus suggests a cis-regulatory role of noncoding intronic transcripts on their parent coding genes.
Copyright © 2013 Elsevier Inc. All rights reserved.
Comment in
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Circuitous route to transcription regulation.Mol Cell. 2013 Sep 26;51(6):705-6. doi: 10.1016/j.molcel.2013.09.012. Mol Cell. 2013. PMID: 24074951 Free PMC article.
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