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. 2014 Jan 21;139(2):439-45.
doi: 10.1039/c3an01835k.

Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general

Shiyun Zhang  1 , Alexa Garcia-D'Angeli, Joseph P Brennan, Qun Huo
Affiliations

Predicting detection limits of enzyme-linked immunosorbent assay (ELISA) and bioanalytical techniques in general

Shiyun Zhang et al. Analyst. .

Abstract

The detection limit is one of the most important performance parameters for bioanalytical techniques. Here we present a generic method to estimate the detection limit of biomolecular assays based on a step-by-step analysis of the assay procedure. Enzyme-linked immunosorbent assay (ELISA) is used here as an example; however, much of the information presented in this article may be applied to other types of biomolecular assays and analytical techniques. A clear understanding of what affects the detection limit can help researchers to evaluate different bio-analytical techniques properly, and to design better strategies to optimize and achieve the best analytical performance.

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