c-myc and c-fos gene regulation during mouse liver regeneration
- PMID: 2512526
c-myc and c-fos gene regulation during mouse liver regeneration
Abstract
We have examined the expression of c-myc and c-fos proto-oncogenes in regenerating mouse liver, in order to analyse the relative contributions of transcriptional and post-transcriptional regulations in vivo. We show that c-myc and c-fos transcription is induced after partial hepatectomy, and involves common mechanisms. A strong block to transcriptional elongation exists in normal liver, within the first exon of c-myc gene. This block is only slightly relieved during liver regeneration, although transcriptional initiation is transiently increased (4-6 fold). In contrast, transcription initiation of c-fos is induced while the transcriptional block within the first exon of the gene is almost completely abolished. The steady-state levels of both transcripts increased to high levels (50-100 fold) within 2-6 h after partial hepatectomy, and were maintained for at least 40 h in the case of c-myc. We conclude that post-transcriptional control mechanisms are largely responsible for the dramatic induction of c-myc mRNA in regenerating liver, while c-fos mRNA accumulation is the result of both an increased initiation and a relief of a transcriptional block to elongation. Induction of both genes in vivo with cycloheximide argues in favor of negative trans-acting proteins which regulate initiation and elongation of transcription.
Similar articles
-
c-myc, c-fos, and c-jun regulation in the regenerating livers of normal and H-2K/c-myc transgenic mice.Mol Cell Biol. 1990 Jun;10(6):3185-93. doi: 10.1128/mcb.10.6.3185-3193.1990. Mol Cell Biol. 1990. PMID: 2111449 Free PMC article.
-
Differential regulation and expression of jun, c-fos and c-myc proto-oncogenes during mouse liver regeneration and after inhibition of protein synthesis.Oncogene. 1990 Oct;5(10):1511-9. Oncogene. 1990. PMID: 2123531
-
Mode of c-myc gene regulation in folic acid-induced kidney regeneration.Oncogene Res. 1989;5(1):67-72. Oncogene Res. 1989. PMID: 2476708
-
c-fos protooncogene expression and neuronal plasticity.Acta Neurobiol Exp (Wars). 1990;50(4-5):173-9. Acta Neurobiol Exp (Wars). 1990. PMID: 2130639 Review.
-
Regulation of c-myc and c-fos proto-oncogene expression by animal cell growth factors.In Vitro Cell Dev Biol. 1988 Feb;24(2):81-4. doi: 10.1007/BF02623883. In Vitro Cell Dev Biol. 1988. PMID: 3125143 Review.
Cited by
-
Negative energy balance and hepatic gene expression patterns in high-yielding dairy cows during the early postpartum period: a global approach.Physiol Genomics. 2010 Nov 15;42A(3):188-99. doi: 10.1152/physiolgenomics.00118.2010. Epub 2010 Aug 17. Physiol Genomics. 2010. PMID: 20716645 Free PMC article.
-
Sequence requirements for premature transcription arrest within the first intron of the mouse c-fos gene.Mol Cell Biol. 1991 May;11(5):2832-41. doi: 10.1128/mcb.11.5.2832-2841.1991. Mol Cell Biol. 1991. PMID: 1901950 Free PMC article.
-
Both coding exons of the c-myc gene contribute to its posttranscriptional regulation in the quiescent liver and regenerating liver and after protein synthesis inhibition.Mol Cell Biol. 1995 Aug;15(8):4410-9. doi: 10.1128/MCB.15.8.4410. Mol Cell Biol. 1995. PMID: 7623834 Free PMC article.
-
c-myc, c-fos, and c-jun regulation in the regenerating livers of normal and H-2K/c-myc transgenic mice.Mol Cell Biol. 1990 Jun;10(6):3185-93. doi: 10.1128/mcb.10.6.3185-3193.1990. Mol Cell Biol. 1990. PMID: 2111449 Free PMC article.
-
Molecular characterization of the in vivo alkylating agent resistant murine EMT-6 mammary carcinoma tumors.Cancer Chemother Pharmacol. 1995;35(5):423-31. doi: 10.1007/s002800050257. Cancer Chemother Pharmacol. 1995. PMID: 7850925