Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1989 Jun 26;17(12):4713-30.
doi: 10.1093/nar/17.12.4713.

Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes

A E Gorbalenya  1 , E V Koonin, A P Donchenko, V M Blinov
Affiliations
Free PMC article
Comparative Study

Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes

A E Gorbalenya et al. Nucleic Acids Res. .
Free PMC article

Abstract

In the course of systematic analysis of protein sequences containing the purine NTP-binding motif, a new superfamily was delineated which included 25 established or putative helicases of Escherichia coli, yeast, insects, mammals, pox- and herpesviruses, a yeast mitochondrial plasmid and three groups of positive strand RNA viruses. These proteins contained 7 distinct highly conserved segments two of which corresponded to the "A" and "B" sites of the NTP-binding motif. Typical of the new superfamily was an abridged consensus for the "A" site, GxGKS/T, instead of the classical G/AxxxxGKS/T. Secondary structure predictions indicated that each of the conserved segments might constitute a separate structural unit centering at a beta-turn. All previously characterized mutations impairing the function of the yeast helicase RAD3 in DNA repair mapped to one of the conserved segments. A degree of similarity was revealed between the consensus pattern of conserved amino acid residues derived for the new superfamily and that of another recently described protein superfamily including a different set of prokaryotic, eukaryotic and viral (putative) helicases.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Virol. 1988 Feb;62(2):444-53 - PubMed
    1. Nucleic Acids Res. 1988 Jan 11;16(1):291-303 - PubMed
    1. J Mol Biol. 1971 Oct 28;61(2):409-24 - PubMed
    1. Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148 - PubMed
    1. Annu Rev Biochem. 1981;50:233-60 - PubMed

Publication types

LinkOut - more resources

  NODES
twitter 2