Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Nov 19;527(7578):323-8.
doi: 10.1038/nature16057. Epub 2015 Nov 4.

Novel antibody-antibiotic conjugate eliminates intracellular S. aureus

Sophie M Lehar  1 , Thomas Pillow  2 , Min Xu  3 , Leanna Staben  2 , Kimberly K Kajihara  1 , Richard Vandlen  4 , Laura DePalatis  4 , Helga Raab  4 , Wouter L Hazenbos  1 , J Hiroshi Morisaki  1 , Janice Kim  3 , Summer Park  3 , Martine Darwish  4 , Byoung-Chul Lee  4 , Hilda Hernandez  5 , Kelly M Loyet  5 , Patrick Lupardus  6 , Rina Fong  6 , Donghong Yan  3 , Cecile Chalouni  7 , Elizabeth Luis  4 , Yana Khalfin  5 , Emile Plise  8 , Jonathan Cheong  8 , Joseph P Lyssikatos  2 , Magnus Strandh  9 , Klaus Koefoed  9 , Peter S Andersen  9 , John A Flygare  2 , Man Wah Tan  1 , Eric J Brown  1 , Sanjeev Mariathasan  1
Affiliations

Novel antibody-antibiotic conjugate eliminates intracellular S. aureus

Sophie M Lehar et al. Nature. .

Abstract

Staphylococcus aureus is considered to be an extracellular pathogen. However, survival of S. aureus within host cells may provide a reservoir relatively protected from antibiotics, thus enabling long-term colonization of the host and explaining clinical failures and relapses after antibiotic therapy. Here we confirm that intracellular reservoirs of S. aureus in mice comprise a virulent subset of bacteria that can establish infection even in the presence of vancomycin, and we introduce a novel therapeutic that effectively kills intracellular S. aureus. This antibody-antibiotic conjugate consists of an anti-S. aureus antibody conjugated to a highly efficacious antibiotic that is activated only after it is released in the proteolytic environment of the phagolysosome. The antibody-antibiotic conjugate is superior to vancomycin for treatment of bacteraemia and provides direct evidence that intracellular S. aureus represents an important component of invasive infections.

PubMed Disclaimer

Comment in

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Haematologica. 2003 Aug;88(8):923-30 - PubMed
    1. J Exp Med. 1952 Feb;95(2):209-30 - PubMed
    1. Proc Natl Acad Sci U S A. 2012 Jul 24;109(30):12147-52 - PubMed
    1. J Immunol. 2000 Apr 1;164(7):3713-22 - PubMed
    1. Antimicrob Agents Chemother. 1979 Dec;16(6):743-9 - PubMed

Publication types

MeSH terms

Associated data

  NODES
Bugs 1
twitter 2