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. 2017 Jan-Mar;40(1):134-141.
doi: 10.1590/1678-4685-GMB-2016-0159. Epub 2017 Mar 20.

Vegetable and fruit juice enhances antioxidant capacity and regulates antioxidant gene expression in rat liver, brain and colon

Linhong Yuan  1 , Jinmeng Liu  1 , Jie Zhen  1 , Yao Xu  1 , Shuying Chen  1 , Nicholas Van Halm-Lutterodt  1 , Rong Xiao  1
Affiliations

Vegetable and fruit juice enhances antioxidant capacity and regulates antioxidant gene expression in rat liver, brain and colon

Linhong Yuan et al. Genet Mol Biol. 2017 Jan-Mar.

Abstract

To explore the effect of fruit and vegetable (FV) juice on biomarkers of oxidative damage and antioxidant gene expression in rats, 36 adult male Wistar rats were randomly divided into control, low FV juice dosage or high FV juice dosage treatment groups. The rats were given freshly extracted FV juice or the same volume of saline water daily for five weeks. After intervention, serum and tissues specimens were collected for biomarker and gene expression measurement. FV juice intervention increased total antioxidant capacity, glutathione, vitamin C, β-carotene, total polyphenols, flavonoids levels andglutathione peroxidaseenzyme activity in rat serum or tissues (p < 0.05). FV juice intervention caused reduction of malondialdehyde levels in rat liver (p < 0.05) and significantly modulated transcript levels of glutamate cysteine ligase catalytic subunit (GCLC) and NAD(P)H:quinone oxidoreductase l (NQO1)in rat liver and brain (p < 0.05). The results underline the potential of FV juice to improve the antioxidant capacity and to prevent the oxidative damage in liver, brain and colon.

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Figures

Figure 1
Figure 1. Expression of antioxidant genes in rat liver (n = 12). Rats received saline water (control), low fruit and vegetable (FV) juice dosage, and high FV juice dosage for 5 weeks. The animals were sacrificed and liver samples were collected for _target gene expression detection by RT-PCR method. β-actin was used as endogenous reference gene, and every experiment was repeated for 3 times. Values represent relative band density (normalized to the endogenous reference gene β-actin). *p < 0.01 compared with control group; *P < .05 compared with low dosage FV juice group.
Figure 2
Figure 2. Expression of antioxidant genes in rat brain (n = 12). Rats received saline water (control), low fruit and vegetable (FV) juice dosage, and high FV juice dosage for 5 weeks. The animals were sacrificed and brain samples were collected for _target gene expression detection by RT-PCR method. β-actin was used as endogenous reference gene, and every experiment was repeated for three times. Values represent relative band density (normalized to the endogenous reference gene β-actin). *p < 0.05 compared with control group.
Figure 3
Figure 3. Expression of antioxidant genes in rat colon (n = 12). Rats received saline water (control), low fruit and vegetable (FV) juice dosage, and high FV juice dosage for 5 weeks. The animals were sacrificed and colon samples were collected for _target gene expression detection by RT-PCR method. β-actin was used as endogenous reference gene, and every experiment was repeated for three times. Values represent relative band density (normalized to the endogenous reference gene β-actin).
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