Modulation of IL-1β and VEGF expression in rat diabetic retinopathy after PACAP administration

doi:10.1016/j.peptides.2017.09.014

. 2017 Nov:97:64-69.

doi: 10.1016/j.peptides.2017.09.014. Epub 2017 Sep 28.

Modulation of IL-1β and VEGF expression in rat diabetic retinopathy after PACAP administration

Agata Grazia D'Amico¹, Grazia Maugeri², Daniela Maria Rasà², Claudio Bucolo³, Salvatore Saccone⁴, Concetta Federico⁴, Sebastiano Cavallaro⁵, Velia D'Agata⁶

Affiliations

¹ Department of Human Science and Promotion of Quality of Life, San Raffaele Open University of Rome, Italy; Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
² Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
³ Section of Pharmacology, Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy; Center for Research in Ocular Pharmacology - CERFO University of Catania, Catania, Italy.
⁴ Section of Animal Biology, Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy.
⁵ Institute of Neurological Sciences, Italian National Research Council, Catania, Italy.
⁶ Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy; Section of Pharmacology, Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy. Electronic address: vdagata@unict.it.

PMID: 28964802
DOI: 10.1016/j.peptides.2017.09.014

Modulation of IL-1β and VEGF expression in rat diabetic retinopathy after PACAP administration

Agata Grazia D'Amico et al. Peptides. 2017 Nov.

. 2017 Nov:97:64-69.

doi: 10.1016/j.peptides.2017.09.014. Epub 2017 Sep 28.

Authors

Agata Grazia D'Amico¹, Grazia Maugeri², Daniela Maria Rasà², Claudio Bucolo³, Salvatore Saccone⁴, Concetta Federico⁴, Sebastiano Cavallaro⁵, Velia D'Agata⁶

Affiliations

¹ Department of Human Science and Promotion of Quality of Life, San Raffaele Open University of Rome, Italy; Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
² Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
³ Section of Pharmacology, Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy; Center for Research in Ocular Pharmacology - CERFO University of Catania, Catania, Italy.
⁴ Section of Animal Biology, Department of Biological, Geological and Environmental Sciences, University of Catania, Catania, Italy.
⁵ Institute of Neurological Sciences, Italian National Research Council, Catania, Italy.
⁶ Section of Human Anatomy and Histology, Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy; Section of Pharmacology, Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy. Electronic address: vdagata@unict.it.

PMID: 28964802
DOI: 10.1016/j.peptides.2017.09.014

Abstract

Diabetic retinopathy (DR) is a microvascular complication of diabetes. Hyperglycemic/hypoxic microenvironment concurs to aberrant angiogenesis characterizing the pathology and activates many downstream _target genes including inflammatory cytokines and vasoactive peptides, such as interleukin-1β (IL-1β) and vascular endothelial growth factor (VEGF). It has been largely demonstrated that pituitary adenylate cyclase-activating peptide (PACAP) plays a protective effect in DR. In the present study, we investigated the role of PACAP to protect retinal tissue through IL-1β and VEGF expression. Diabetes was induced in rats by streptozotocin (STZ) injection, and one week later a single intravitreal injection of 100μM PACAP was administrated. Analyses of IL-1β and VEGF levels were performed three weeks after diabetes induction. The results demonstrated that a single intraocular administration of PACAP significantly reduced the expression of IL-1β in diabetic animals. Moreover, it affects VEGF and its receptors (VEGFRs) levels and interferes with their retinal layers distribution as showed by confocal microscopy analysis. In particular, PACAP treatment downregulates VEGF and VEGFRs that are increasingly expressed in STZ-treated animals as compared to controls. These results indicate that PACAP plays an important role to attenuate the early phase of DR.

Keywords: Diabetic retinopathy; Interleukin-1β; PACAP; VEGF.

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Modulation of IL-1β and VEGF expression in rat diabetic retinopathy after PACAP administration

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Modulation of IL-1β and VEGF expression in rat diabetic retinopathy after PACAP administration

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