Antibodies specific for N6-methyladenosine react with intact snRNPs U2 and U4/U6
- PMID: 2951275
- DOI: 10.1016/0014-5793(87)81512-0
Antibodies specific for N6-methyladenosine react with intact snRNPs U2 and U4/U6
Abstract
Antibodies specific for N6-methyladenosine (m6A) were elicited in rabbits and used to study the accessibility in intact snRNPs of the m6A residues present in the snRNAs U2, U4 and U6. The antibody quantitatively precipitates snRNPs U2 and U4/U6 from total nucleoplasmic snRNPs U1-U6 isolated from HeLa cells, which demonstrates that the m6A residues of the respective snRNAs are not protected by snRNP proteins in the snRNP particles. While the anti-m6A IgG does not react at all with U5 RNPs lacking m6A, a significant amount of U1 RNPs was co-precipitated despite the fact that U1 RNA does not contain m6A either. Since anti-m6A IgG does not react with purified U1 RNPs and co-precipitation of U1 RNPs is dependent on the presence of U2 RNPs but not of U4/U6 RNPs, these data indicate an interaction between snRNPs U1 and U2 in vitro. The anti-m6A precipitation pattern described above was also observed with snRNPs isolation from mouse Ehrlich ascites tumor cells, indicating similar three-dimensional arrangements of snRNAs in homologous snRNP particles from different organisms.
Similar articles
-
Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents.EMBO J. 1986 Dec 20;5(13):3509-16. doi: 10.1002/j.1460-2075.1986.tb04676.x. EMBO J. 1986. PMID: 2951249 Free PMC article.
-
Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing.EMBO J. 1984 Jun;3(6):1357-63. doi: 10.1002/j.1460-2075.1984.tb01977.x. EMBO J. 1984. PMID: 6204860 Free PMC article.
-
Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera.EMBO J. 1983;2(7):1129-35. doi: 10.1002/j.1460-2075.1983.tb01557.x. EMBO J. 1983. PMID: 6194992 Free PMC article.
-
Autoantibodies reactive with small ribonucleoprotein antigens: a convergence of molecular biology and clinical immunology.J Clin Lab Immunol. 1984 Sep;15(1):1-17. J Clin Lab Immunol. 1984. PMID: 6210367 Review.
-
Particles containing small molecular weight nuclear RNAs (snRNPs). Structure and possible functions.Mol Biol Rep. 1981 May 22;7(1-3):41-5. doi: 10.1007/BF00778731. Mol Biol Rep. 1981. PMID: 6166852 Review.
Cited by
-
N6-methyl-adenosine (m6A) in RNA: an old modification with a novel epigenetic function.Genomics Proteomics Bioinformatics. 2013 Feb;11(1):8-17. doi: 10.1016/j.gpb.2012.12.002. Epub 2012 Dec 21. Genomics Proteomics Bioinformatics. 2013. PMID: 23453015 Free PMC article. Review.
-
U1-U2 snRNPs interaction induced by an RNA complementary to the 5' end sequence of U1 snRNA.Nucleic Acids Res. 1992 Jul 25;20(14):3625-30. doi: 10.1093/nar/20.14.3625. Nucleic Acids Res. 1992. PMID: 1386423 Free PMC article.
-
Chemical Modifications in the Life of an mRNA Transcript.Annu Rev Genet. 2018 Nov 23;52:349-372. doi: 10.1146/annurev-genet-120417-031522. Epub 2018 Sep 19. Annu Rev Genet. 2018. PMID: 30230927 Free PMC article. Review.
-
RNA methylation in nuclear pre-mRNA processing.Wiley Interdiscip Rev RNA. 2018 Nov;9(6):e1489. doi: 10.1002/wrna.1489. Epub 2018 Jun 19. Wiley Interdiscip Rev RNA. 2018. PMID: 29921017 Free PMC article. Review.
-
The life of U6 small nuclear RNA, from cradle to grave.RNA. 2018 Apr;24(4):437-460. doi: 10.1261/rna.065136.117. Epub 2018 Jan 24. RNA. 2018. PMID: 29367453 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
