Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Apr 9;29(4):45.
doi: 10.1007/s10856-018-6051-7.

Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone

M Leonhard  1 , B Zatorska  2 , D Moser  3 , Y Tan  2 , B Schneider-Stickler  2
Affiliations

Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone

M Leonhard et al. J Mater Sci Mater Med. .

Abstract

In the upper aerodigestive tract, biofilm deposits by oropharyngeal microbes can cause failure of medical polymer devices like voice prostheses. Previous studies on testing of inhibitive strategies still lack of comparability due to varying study protocols concerning growth media, microbial species and growth conditions. Goal of the study was therefore to test cultivation of a mixed biofilm of isolated oropharyngeal microbes under in vitro growth conditions using mixtures of common growth media. Mixtures of yeast peptone dextrose medium (YPD), fetal bovine serum (FBS), RPMI 1640, Yeast nitrogen base medium (YNB) and brain heart infusion (BHI) were tested to grow mixed biofilm deposits of Candida albicans, Candida tropicalis, Staphylococcus aureus, Streptococcus epidermidis, Rothia dentocariosa and Lactobacillus gasseri on medical grade silicone. Periodic assessment of living biofilm was performed over 22 days by a digital microscope and the cultivated biofilm structures were analyzed by scanning electron microscopy after completion of the study. Mixtures of BHI, YPD and FBS improved microscopic growth of multispecies biofilm deposits over time, while addition of RPMI and YNB resulted in reduction of visible biofilm deposit sizes. A mixtures of FBS 30% + YPD 70% and BHI 30% + YPD 70% showed enhanced support of permanent surface growth on silicone. Growth kinetics of in vitro multispecies biofilms can be manipulated by using mixtures of common growth media. Using mixtures of growth media can improve growth of longterm multispecies oropharyngeal biofilm models used for in vitro testing of antibiofilm materials or coatings for voice prostheses.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
a View of a dysfunctional Provox 2 voice prosthesis at explantation after 96 days in situ. The valve flap and esophageal flange are overgrown by candida based mixed biofilm deposits. Transprosthetic leakage of esophageal contents is caused by impairment of the valve closure due to biofilm deposits and deterioration of the silicone material. b Candida species infiltrate the silicone material of a VP after weeks (cross section of a Provox 2 after 48 weeks in situ, white arrow: site of biofilm infiltration, shaped arrow: surface of the silicone material)
Fig. 2
Fig. 2
Scheme of the applied microtiter biofilm model and quantification of macroscopic size of biofilm deposits using image analysis software. The size of biofilm cover was calculated as percentage of each total platelet surface. (Artwork created with Inkscape 0.91)
Fig. 3
Fig. 3
Overview of growth kinetics of biofilm deposits over 22 days in the tested growth media: yeast peptone dextrose (YPD), fetal bovine serum (FBS) and mixtures with brain heart infusion (BHI), yeast nitrogen base (YNB) and RPMI 1640 (RPMI)
Fig. 4
Fig. 4
Scanning electron micrographs of the resulting biofilm structures assessed on silicone after 22 days in the in vitro biofilm model. Key structures of biofilms on explanted voice prostheses, such as budded yeast, hypheal germination and balanced bacterial proliferation were assessed in all growth media tested, except in the mixture of FBS 30% + YNB 70%
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Lorenz KJ. Voice rehabilitation after total laryngectomy: a chronological review of medical history. HNO. 2015;63:663–4–666–80. doi: 10.1007/s00106-015-0043-4. - DOI - PubMed
    1. Kojic EM, Darouiche RO. Candida infections of medical devices. Clin Microbiol Rev. 2004;17:255–67. doi: 10.1128/CMR.17.2.255-267.2004. - DOI - PMC - PubMed
    1. Bauters TGM, Moerman M, Vermeersch H, Nelis HJ. Colonization of voice prostheses by albicans and non-albicans Candida species. Laryngoscope. 2002;112:708–12. doi: 10.1097/00005537-200204000-00021. - DOI - PubMed
    1. Tićac B, Tićac R, Rukavina T, Kesovija PG, Pedisić D, Maljevac B, Starčević R. Microbial colonization of tracheoesophageal voice prostheses (Provox2) following total laryngectomy. Eur Arch Otorhinolaryngol. 2010;267:1579–86. doi: 10.1007/s00405-010-1253-8. - DOI - PubMed
    1. Lewin JS, Baumgart LM, Barrow MP, Hutcheson KA. Device life of the tracheoesophageal voice prosthesis revisited. JAMA Otolaryngol Head Neck Surg. 2017;143:65–71. doi: 10.1001/jamaoto.2016.2771. - DOI - PMC - PubMed

LinkOut - more resources

  NODES
twitter 2