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. 2018 Mar 15;13(T-NANO 2014 Abstracts):43-46.
doi: 10.2147/IJN.S125002. eCollection 2018.

Preparation and characterization of superparamagnetic iron oxide nanoparticles for magnetically guided drug delivery

Pramod Kumar  1 , Shrish Agnihotri  1 , Indrajit Roy  1
Affiliations

Preparation and characterization of superparamagnetic iron oxide nanoparticles for magnetically guided drug delivery

Pramod Kumar et al. Int J Nanomedicine. .

Abstract

Iron oxide nanoparticles have unique magnetic properties and therefore readily respond to applied magnetic fields. Moreover, their surfaces can be used to attach active molecules via various covalent or noncovalent interactions. Thus, they can be used as drug carriers for magnetically controlled delivery to specific biological sites of interest. In the present study, we have synthesized aqueous dispersed samples of citric acid-capped iron oxide nanoparticles, and the anticancer drug doxorubicin was then linked with these superparamagnetic iron oxide nanoparticles via a simple noncovalent interaction. Our results show that the conjugated drug releases from the nanoparticles in a sustained manner. The cellular uptake of these nanoparticles was found to be substantial, although it can be further enhanced using magnetic guidance. These nanoparticles (drug free) were found to be nontoxic to cells; however, upon drug conjugation, drug-induced toxicity was observed, owing to the slow release of drug from the nanoparticles.

Keywords: Dox; Dox-CA-SPION; SPION; magnetically guided delivery.

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Conflict of interest statement

Disclosure The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Transmission electron microscopy pictures of CA-SPION, showing their uniformity of size. Abbreviation: CA-SPION, citric acid coated superparamagnetic iron oxide nanoparticles.
Figure 2
Figure 2
(A) Hemolysis and (B) cell viability result of SPION and CA-SPION. Abbreviations: SPION, superparamagnetic iron oxide nanoparticles; Conc, concentration; CA-SPION, citric acid coated SPION; TX-100, Triton-X-100.
Figure 3
Figure 3
(A) Estimation of total intracellular iron content from cells treated with Dox-CA-SPION, with and without magnetic guidance, (B) estimation of intracellular Dox-fluorescence from cells treated with Dox-CA-SPION, and (C) cell viability assay results of DOX-CA-SPION, with and without magnetic guidance. Note: Treatment times were 5 and 15 minutes in each case. Abbreviations: Conc, concentration; Dox-CA-SPION, doxorubicin containing citric acid-coated SPION; SPION, superparamagnetic iron oxide nanoparticles.
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