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. 2019 Apr;9(4):138.
doi: 10.1007/s13205-019-1654-3. Epub 2019 Mar 12.

Distribution of antibiotic resistance and virulence factors among the bacteria isolated from diseased Etroplus suratensis

Aswani Ravi #  1 , Soumya Das #  1 , Jasim Basheer  1 , Aswathy Chandran  2 , Chinnu Benny  2 , Sindhura Somaraj  2 , Sebastian Korattiparambil Sebastian  3 , Jyothis Mathew  1 , Radhakrishnan Edayileveettil Krishnankutty  1
Affiliations

Distribution of antibiotic resistance and virulence factors among the bacteria isolated from diseased Etroplus suratensis

Aswani Ravi et al. 3 Biotech. 2019 Apr.

Abstract

Considering the emerging concern with the antimicrobial resistance (AMR) evolution, the study has been designed to identify the antibiotic resistance and virulence properties of culturable bacteria isolated from the diseased fish Etroplus suratensis. This has resulted in the purification of 18 morphologically distinct bacterial isolates which were identified by both biochemical and molecular methods. Antibiotic resistance analysis showed the resistance of these isolates to multiple antibiotics and remarkable evolution of AMR. Further screening for virulence factors confirmed five isolates to be positive for haemolytic activity, eight with caseinase, four with DNase, one with gelatinase and three with biofilm-forming properties. In addition to these, the isolates were subjected to PCR-based screening to detect the presence of genes coding for aerolysin and haemolysin. Results showed the presence aerolysin gene in the isolates ESS3.2, ESS3.8, ESI3.3 and ESS3.6, while haemolysin gene was observed to be present in ESG3.1 and ESI3.2. The observed results hence indicate the need for frequent monitoring of these properties among bacterial isolates from diverse environment especially those associated with edible fish.

Keywords: Antimicrobial resistance; Aquaculture; Etroplus suratensis; Virulence mechanisms.

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Conflict of interest statement

Compliance with ethical standardsThe authors declared that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
PCR amplification of 16 SrDNA from selected bacterial isolates. Lanes 1–9 represent 1500 bp product from the isolates ESS3.1, ESS3.2, ESS3.3, ESS3.4, ESS3.5, ESS3.6, ESS3.7, ESG3.1, and ESG3.2. Lane M represents 100 bp ladder and Lane 10–18 represent 1500 bp product from ESG3.3, ESI3.1, ESI3.2 and ESI3.3, ESW3.1, ESW3.2, ESW3.3, ESW3.4 and ESW3.5
Fig. 2
Fig. 2
The phylogenetic relationship of the bacterial isolates obtained from E. suratensis
Fig. 3
Fig. 3
PCR gel image of haemolysin gene with a product size of 1320 bp. Lane 1—ESG3.1; Lane 2—ESI3.2; Lane M—Marker
Fig. 4
Fig. 4
PCR gel image of aerolysin gene with a product size of 431 bp; Lane M—Marker; Lane 1—ESS3.2; Lane 2—ESS3.8; Lane 3—ESI3.3; and Lane 4—ESS3.6
Fig. 5
Fig. 5
Antibiotic resistance, sensitivity and intermediate resistance of the obtained isolates
Fig. 6
Fig. 6
Graphical representation of biofilm formation in bacterial isolates from diseased E. suratensis
See this image and copyright information in PMC

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