Simultaneous _targeted Detection of Proteins and RNAs in Single Cells
- PMID: 31028649
- DOI: 10.1007/978-1-4939-9240-9_22
Simultaneous _targeted Detection of Proteins and RNAs in Single Cells
Abstract
Simultaneous detection of both RNA and protein in individual single cells offers a powerful tool for genotype-to-phenotype investigations. Proximity extension assay (PEA) is a quantitative, sensitive, and multiplex protein detection system that has superb utility in single-cell omic analysis. We implemented PEA using the flexible microfluidic workflow of the Fluidigm® C1™ system followed by real-time quantitative polymerase chain reaction (RT-qPCR) on the Fluidigm Biomark™ HD system. With this workflow, _targeted quantification of RNAs and proteins within individual cells is readily conducted.
Keywords: Antibody detection; Microfluidics; Multi-omics; Protein quantification; Protein–RNA correlation; Proximity extension assay (PEA); RT-qPCR; Single-cell analysis; Single-cell protein detection; qPCR.
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