I kappa B: a specific inhibitor of the NF-kappa B transcription factor
- PMID: 3140380
- DOI: 10.1126/science.3140380
I kappa B: a specific inhibitor of the NF-kappa B transcription factor
Abstract
In cells that do not express immunoglobulin kappa light chain genes, the kappa enhancer binding protein NF-kappa B is found in cytosolic fractions and exhibits DNA binding activity only in the presence of a dissociating agent such as sodium deoxycholate. The dependence on deoxycholate is shown to result from association of NF-kappa B with a 60- to 70-kilodalton inhibitory protein (I kappa B). The fractionated inhibitor can inactivate NF-kappa B from various sources--including the nuclei of phorbol ester-treated cells--in a specific, saturable, and reversible manner. The cytoplasmic localization of the complex of NF-kappa B and I kappa B was supported by enucleation experiments. An active phorbol ester must therefore, presumably by activation of protein kinase C, cause dissociation of a cytoplasmic complex of NF-kappa B and I kappa B by modifying I kappa B. this releases active NF-kappa B which can translocate into the nucleus to activate _target enhancers. The data show the existence of a phorbol ester-responsive regulatory protein that acts by controlling the DNA binding activity and subcellular localization of a transcription factor.
Similar articles
-
Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor.Cell. 1988 Apr 22;53(2):211-7. doi: 10.1016/0092-8674(88)90382-0. Cell. 1988. PMID: 3129195
-
The NF-kappa B-binding site mediates phorbol ester-inducible transcription in nonlymphoid cells.Mol Cell Biol. 1988 Aug;8(8):3526-31. doi: 10.1128/mcb.8.8.3526-3531.1988. Mol Cell Biol. 1988. PMID: 3145412 Free PMC article.
-
PMA/ionomycin induces Ig kappa 3' enhancer activity which is in part mediated by a unique NFAT transcription complex.Eur J Immunol. 1998 May;28(5):1467-80. doi: 10.1002/(SICI)1521-4141(199805)28:05<1467::AID-IMMU1467>3.0.CO;2-9. Eur J Immunol. 1998. PMID: 9603451
-
[Nuclear transcription factor kappa B (NF-kappa B)].Rev Med Interne. 1998 Dec;19(12):945-7. doi: 10.1016/s0248-8663(99)80074-5. Rev Med Interne. 1998. PMID: 9887469 Review. French.
-
NF-kappa B: a pleiotropic mediator of inducible and tissue-specific gene control.Cell. 1989 Jul 28;58(2):227-9. doi: 10.1016/0092-8674(89)90833-7. Cell. 1989. PMID: 2665943 Review. No abstract available.
Cited by
-
Modulation of Macrophage Inflammatory Nuclear Factor κB (NF-κB) Signaling by Intracellular Cryptococcus neoformans.J Biol Chem. 2016 Jul 22;291(30):15614-27. doi: 10.1074/jbc.M116.738187. Epub 2016 May 26. J Biol Chem. 2016. PMID: 27231343 Free PMC article.
-
Angiotensin-converting enzyme 2/angiotensin-(1-7)/Mas axis prevents lipopolysaccharide-induced apoptosis of pulmonary microvascular endothelial cells by inhibiting JNK/NF-κB pathways.Sci Rep. 2015 Feb 3;5:8209. doi: 10.1038/srep08209. Sci Rep. 2015. PMID: 25644821 Free PMC article.
-
The multifunctional role of intrinsic disorder in NF-κB signaling.Biochem Soc Trans. 2023 Dec 20;51(6):2085-2092. doi: 10.1042/BST20230035. Biochem Soc Trans. 2023. PMID: 38095058 Free PMC article. Review.
-
Interaction of an NF-kappa B-like factor with a site upstream of the c-myc promoter.Proc Natl Acad Sci U S A. 1990 Jun;87(12):4727-31. doi: 10.1073/pnas.87.12.4727. Proc Natl Acad Sci U S A. 1990. PMID: 2191300 Free PMC article.
-
Cyclic AMP-independent activation of transcription factor NF-kappa B in HL60 cells by tumor necrosis factors alpha and beta.Mol Cell Biol. 1991 Apr;11(4):2315-8. doi: 10.1128/mcb.11.4.2315-2318.1991. Mol Cell Biol. 1991. PMID: 1706475 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
