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. 2020 Jul;40(7):3819-3830.
doi: 10.21873/anticanres.14371.

Anti-tumor Properties of Picrasma quassioides Extracts in H-RasG12V Liver Cancer Are Mediated Through ROS-dependent Mitochondrial Dysfunction

Dan-Ping Xie  1 , Yi-Xi Gong  1 , Ying-Hua Jin  2 , Chen-Xi Ren  1 , Yue Liu  1 , Ying-Hao Han  1 , Mei-Hua Jin  1 , Dan Zhu  1 , Qiu-Zhen Pan  1 , Li-Yun Yu  1 , Dong-Seok Lee  3 , Jaihyung Lee  4 , Jihwan Kim  5 , Yang Ho Park  6 , Jin Won Hyun  7 , Taeho Kwon  8 , Yu-Dong Cui  9 , Hu-Nan Sun  9
Affiliations

Anti-tumor Properties of Picrasma quassioides Extracts in H-RasG12V Liver Cancer Are Mediated Through ROS-dependent Mitochondrial Dysfunction

Dan-Ping Xie et al. Anticancer Res. 2020 Jul.

Abstract

Background: Picrasma quassioides (PQ) is a traditional Asian herbal medicine with anti-tumor properties that can inhibit the viability of HepG2 liver cancer cells. H-Ras is often mutated in liver cancer, however, the effect of PQ treatment on H-Ras mutated liver cancer is unclear. This study aimed to investigate the role of PQ on ROS accumulation and mitochondrial dysfunction in H-ras mutated HepG2 (HepG2G12V) cells.

Materials and methods: PQ ethanol extract-induced HepG2G12V apoptosis was analyzed by the MTT assay, fluorescence microscopy, flow cytometry and western blotting.

Results: PQ treatment affected cell migration and colony formation in HepG2G12V cells. Cleaved-caspase-3, cleaved-caspase-9 and BCL2 associated agonist of cell death (BAD) expression levels were increased, while the levels of B-cell lymphoma-extra large (Bcl-xL) were decreased with PQ treatment. PQ treatment led to a reduction of H-Ras expression levels in liver cancer cells, thus reducing their abnormal proliferation. Furthermore, it led to increased expression levels of Peroxiredoxin VI, which regulates the redox signal in cells.

Conclusion: Taken together these results provide a new functional significance for the role of PQ in treating HepG2G12V liver cancer.

Keywords: Liver cancer; Picrasma quassioides; ROS; mitochondria.

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