An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs
- PMID: 3313277
- PMCID: PMC306349
- DOI: 10.1093/nar/15.20.8125
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs
Abstract
5'-Noncoding sequences have been compiled from 699 vertebrate mRNAs. (GCC) GCCA/GCCATGG emerges as the consensus sequence for initiation of translation in vertebrates. The most highly conserved position in that motif is the purine in position -3 (three nucleotides upstream from the ATG codon); 97% of vertebrate mRNAs have a purine, most often A, in that position. The periodical occurrence of G (in positions -3, -6, -9) is discussed. Upstream ATG codons occur in fewer than 10% of vertebrate mRNAs-at-large; a notable exception are oncogene transcripts, two-thirds of which have ATG codons preceding the start of the major open reading frame. The leader sequences of most vertebrate mRNAs fall in the size range of 20 to 100 nucleotides. The significance of shorter and longer 5'-noncoding sequences is discussed.
Similar articles
-
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.Nucleic Acids Res. 1984 Jan 25;12(2):857-72. doi: 10.1093/nar/12.2.857. Nucleic Acids Res. 1984. PMID: 6694911 Free PMC article.
-
Quantification analysis of translation initiation signal in vertebrate mRNAs: effect of nucleotides at positions +4(-)+6 upon efficiency of translation initiation.Nucleic Acids Symp Ser. 2000;(44):77-8. doi: 10.1093/nass/44.1.77. Nucleic Acids Symp Ser. 2000. PMID: 12903276
-
Interpreting cDNA sequences: some insights from studies on translation.Mamm Genome. 1996 Aug;7(8):563-74. doi: 10.1007/s003359900171. Mamm Genome. 1996. PMID: 8679005 Review.
-
Diversity in G + C content at the third position of codons in vertebrate genes and its cause.Nucleic Acids Res. 1986 Aug 26;14(16):6345-55. doi: 10.1093/nar/14.16.6345. Nucleic Acids Res. 1986. PMID: 3748815 Free PMC article.
-
An analysis of vertebrate mRNA sequences: intimations of translational control.J Cell Biol. 1991 Nov;115(4):887-903. doi: 10.1083/jcb.115.4.887. J Cell Biol. 1991. PMID: 1955461 Free PMC article. Review.
Cited by
-
UPF0586 Protein C9orf41 Homolog Is Anserine-producing Methyltransferase.J Biol Chem. 2015 Jul 10;290(28):17190-205. doi: 10.1074/jbc.M115.640037. Epub 2015 May 22. J Biol Chem. 2015. PMID: 26001783 Free PMC article.
-
mRNA decay during herpes simplex virus (HSV) infections: mutations that affect translation of an mRNA influence the sites at which it is cleaved by the HSV virion host shutoff (Vhs) protein.J Virol. 2013 Jan;87(1):94-109. doi: 10.1128/JVI.01557-12. Epub 2012 Oct 17. J Virol. 2013. PMID: 23077305 Free PMC article.
-
Deep proteome coverage based on ribosome profiling aids mass spectrometry-based protein and peptide discovery and provides evidence of alternative translation products and near-cognate translation initiation events.Mol Cell Proteomics. 2013 Jul;12(7):1780-90. doi: 10.1074/mcp.M113.027540. Epub 2013 Feb 21. Mol Cell Proteomics. 2013. PMID: 23429522 Free PMC article.
-
Expression of human Hemojuvelin (HJV) is tightly regulated by two upstream open reading frames in HJV mRNA that respond to iron overload in hepatic cells.Mol Cell Biol. 2015 Apr;35(8):1376-89. doi: 10.1128/MCB.01462-14. Epub 2015 Feb 9. Mol Cell Biol. 2015. PMID: 25666510 Free PMC article.
-
Cynoglossus semilaevis thioredoxin: a reductase and an antioxidant with immunostimulatory property.Cell Stress Chaperones. 2012 Jul;17(4):445-55. doi: 10.1007/s12192-012-0322-x. Epub 2012 Jan 18. Cell Stress Chaperones. 2012. PMID: 22270611 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
