Epigallocatechin-3-gallate induces autophagy-related apoptosis associated with LC3B II and Beclin expression of bladder cancer cells

doi:10.1111/jfbc.13758

. 2021 Jun;45(6):e13758.

doi: 10.1111/jfbc.13758. Epub 2021 May 16.

Epigallocatechin-3-gallate induces autophagy-related apoptosis associated with LC3B II and Beclin expression of bladder cancer cells

Zhaofa Yin¹, Juan Li², Le Kang¹, Xiangyang Liu³, Jianguo Luo¹, Ling Zhang¹, Yuting Li¹, Jiarong Cai¹

Affiliations

¹ Department of Urology, Loudi Central Hospital of Hunan Province, Loudi, P.R. China.
² Department of Digestive, 2nd Affiliated Hospital of Chongqing Medical University, Chongqing, China.
³ Department of Ultrasound, Loudi Central Hospital of Hunan Province, Loudi, China.

PMID: 33997996
DOI: 10.1111/jfbc.13758

Epigallocatechin-3-gallate induces autophagy-related apoptosis associated with LC3B II and Beclin expression of bladder cancer cells

Zhaofa Yin et al. J Food Biochem. 2021 Jun.

. 2021 Jun;45(6):e13758.

doi: 10.1111/jfbc.13758. Epub 2021 May 16.

Authors

Zhaofa Yin¹, Juan Li², Le Kang¹, Xiangyang Liu³, Jianguo Luo¹, Ling Zhang¹, Yuting Li¹, Jiarong Cai¹

Affiliations

¹ Department of Urology, Loudi Central Hospital of Hunan Province, Loudi, P.R. China.
² Department of Digestive, 2nd Affiliated Hospital of Chongqing Medical University, Chongqing, China.
³ Department of Ultrasound, Loudi Central Hospital of Hunan Province, Loudi, China.

PMID: 33997996
DOI: 10.1111/jfbc.13758

Abstract

The incidence of bladder cancer in traditional green tea-consuming countries was dramatically lower than low green tea-consuming countries. Epigallocatechin-3-gallate (EGCG), an active ingredient extracted from green tea, showed effective inhibition of formation and progression of many tumors. However, whether autophagy involved in this tumor-suppression mechanism of EGCG on bladder cancer was still unclear. In this study, we demonstrated low concentration of EGCG-induced proliferation inhibition and increased apoptosis in bladder cancer cell lines (5,637 and T24 cells) indicated by the increased expression of apoptosis-related protein (caspase9, caspase3 and BAX). In addition, low dose of EGCG also regulated autophagy pathway associated protein (LC3B II and Beclin) expression and this autophagy pathway was blocked by PI3K/AKT inhibitor; moreover, knockdown of ATG5 reversed EGCG-induced apoptosis in 5,637 cells, indicating that EGCG might inhibit the bladder cancer through autophagy pathway. Our findings indicated that EGCG should be considered as a novel therapy for bladder cancer treatment by regulating autophagy pathway. PRACTICAL APPLICATIONS: Our research proved EGCG from green tea could be used as an effective anti-tumor ingredient by revealing another mechanism that epigallocatechin-3-Gallate inhibited bladder cancer cells via inducing autophagy-related apoptosis. And green tea could be considered as a kind of tumor-preventing beverage.

Keywords: apoptosis; autophagy; bladder cancer; epigallocatechin-3-gallate.

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References

REFERENCES

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1. Bimonte, S., & Cascella, M. (2020). The potential roles of epigallocatechin-3-gallate in the treatment of ovarian cancer: current state of knowledge. Drug Design, Development and Therapy, 14, 4245-4250. https://doi.org/10.2147/DDDT.S253092
1. Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68, 394-424. https://doi.org/10.3322/caac.21492
1. Cai, N., Dai, S. D., Liu, N. N., Liu, L. M., Zhao, N., & Chen, L. (2012). PI3K/AKT/mTOR signaling pathway inhibitors in proliferation of retinal pigment epithelial cells. International Journal of Ophthalmology, 5, 675-680. https://doi.org/10.3980/j.issn.2222-3959.2012.06.05
1. Chen, X., Tong, R., Liu, B., Liu, H., Feng, X., Ding, S., Lei, Q., Tang, G., Wu, J., & Fang, W. (2020). Duo of (-)-epigallocatechin-3-gallate and doxorubicin loaded by polydopamine coating ZIF-8 in the regulation of autophagy for chemo-photothermal synergistic therapy. Biomaterials Science, 8, 1380-1393. https://doi.org/10.1039/c9bm01614g

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[1] Amaravadi, R. K., Yu, D., Lum, J. J., Bui, T., Christophorou, M. A., Evan, G. I., Thomas-Tikhonenko, A., & Thompson, C. B. (2007). Autophagy inhibition enhances therapy-induced apoptosis in a Myc-induced model of lymphoma. Journal of Clinical Investigation, 117, 326-336. https://doi.org/10.1172/JCI28833

[2] Amaravadi, R. K., Yu, D., Lum, J. J., Bui, T., Christophorou, M. A., Evan, G. I., Thomas-Tikhonenko, A., & Thompson, C. B. (2007). Autophagy inhibition enhances therapy-induced apoptosis in a Myc-induced model of lymphoma. Journal of Clinical Investigation, 117, 326-336. https://doi.org/10.1172/JCI28833

[3] Bimonte, S., & Cascella, M. (2020). The potential roles of epigallocatechin-3-gallate in the treatment of ovarian cancer: current state of knowledge. Drug Design, Development and Therapy, 14, 4245-4250. https://doi.org/10.2147/DDDT.S253092

[4] Bimonte, S., & Cascella, M. (2020). The potential roles of epigallocatechin-3-gallate in the treatment of ovarian cancer: current state of knowledge. Drug Design, Development and Therapy, 14, 4245-4250. https://doi.org/10.2147/DDDT.S253092

[5] Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68, 394-424. https://doi.org/10.3322/caac.21492

[6] Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68, 394-424. https://doi.org/10.3322/caac.21492

[7] Cai, N., Dai, S. D., Liu, N. N., Liu, L. M., Zhao, N., & Chen, L. (2012). PI3K/AKT/mTOR signaling pathway inhibitors in proliferation of retinal pigment epithelial cells. International Journal of Ophthalmology, 5, 675-680. https://doi.org/10.3980/j.issn.2222-3959.2012.06.05

[8] Cai, N., Dai, S. D., Liu, N. N., Liu, L. M., Zhao, N., & Chen, L. (2012). PI3K/AKT/mTOR signaling pathway inhibitors in proliferation of retinal pigment epithelial cells. International Journal of Ophthalmology, 5, 675-680. https://doi.org/10.3980/j.issn.2222-3959.2012.06.05

[9] Chen, X., Tong, R., Liu, B., Liu, H., Feng, X., Ding, S., Lei, Q., Tang, G., Wu, J., & Fang, W. (2020). Duo of (-)-epigallocatechin-3-gallate and doxorubicin loaded by polydopamine coating ZIF-8 in the regulation of autophagy for chemo-photothermal synergistic therapy. Biomaterials Science, 8, 1380-1393. https://doi.org/10.1039/c9bm01614g

[10] Chen, X., Tong, R., Liu, B., Liu, H., Feng, X., Ding, S., Lei, Q., Tang, G., Wu, J., & Fang, W. (2020). Duo of (-)-epigallocatechin-3-gallate and doxorubicin loaded by polydopamine coating ZIF-8 in the regulation of autophagy for chemo-photothermal synergistic therapy. Biomaterials Science, 8, 1380-1393. https://doi.org/10.1039/c9bm01614g

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Epigallocatechin-3-gallate induces autophagy-related apoptosis associated with LC3B II and Beclin expression of bladder cancer cells

Affiliations

Epigallocatechin-3-gallate induces autophagy-related apoptosis associated with LC3B II and Beclin expression of bladder cancer cells

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