In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps

doi:10.1007/978-1-0716-1162-3_10

. 2021:2255:97-117.

doi: 10.1007/978-1-0716-1162-3_10.

In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps

Guillermina Calo¹, Analia Silvina Trevani², Esteban Grasso³, Irene Angelica Keitelman², Rosanna Ramhorst³, Claudia Pérez Leirós³, Florencia Sabbione²

Affiliations

¹ CONICET-Universidad de Buenos Aires. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Laboratorio de inmunofarmacología, Buenos Aires, Argentina. guillecalo@yahoo.com.ar.
² CONICET-Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX). Laboratorio de Inmunidad Innata, Buenos Aires, Argentina.
³ CONICET-Universidad de Buenos Aires. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Laboratorio de inmunofarmacología, Buenos Aires, Argentina.

PMID: 34033098
DOI: 10.1007/978-1-0716-1162-3_10

In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps

Guillermina Calo et al. Methods Mol Biol. 2021.

. 2021:2255:97-117.

doi: 10.1007/978-1-0716-1162-3_10.

Authors

Guillermina Calo¹, Analia Silvina Trevani², Esteban Grasso³, Irene Angelica Keitelman², Rosanna Ramhorst³, Claudia Pérez Leirós³, Florencia Sabbione²

Affiliations

¹ CONICET-Universidad de Buenos Aires. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Laboratorio de inmunofarmacología, Buenos Aires, Argentina. guillecalo@yahoo.com.ar.
² CONICET-Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX). Laboratorio de Inmunidad Innata, Buenos Aires, Argentina.
³ CONICET-Universidad de Buenos Aires. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN). Laboratorio de inmunofarmacología, Buenos Aires, Argentina.

PMID: 34033098
DOI: 10.1007/978-1-0716-1162-3_10

Abstract

Neutrophils release web like-structures known as neutrophil extracellular traps (NETs) that ensnare and kill microorganisms. These networks are constituted of a DNA scaffold with associated antimicrobial proteins, which are released to the extracellular space as an effective mechanism to fight against invading microorganisms. In parallel with this beneficial role to avoid microbial dissemination and wall off infections, accumulating evidence supports that under certain circumstances, NETs can exert deleterious effects in inflammatory, autoimmune, and thrombotic pathologies. Research on NET properties and their role in pathophysiological processes is a rapidly evolving and expanding field. Here, we describe a combination of methods to achieve a successful in vitro NET visualization, semiquantification, and isolation.

Keywords: Cell death; Elastase; Myeloperoxidase; NETosis; Neutrophil extracellular traps.

PubMed Disclaimer

Cited by

Competitive survival of clonal serial Pseudomonas aeruginosa isolates from cystic fibrosis airways in human neutrophils.
Kuschnerow P, Munder A, de Buhr N, Mörgelin M, Jirmo AC, Ackermann M, von Köckritz-Blickwede M, Tümmler B, Cramer N. Kuschnerow P, et al. iScience. 2023 Mar 24;26(4):106475. doi: 10.1016/j.isci.2023.106475. eCollection 2023 Apr 21. iScience. 2023. PMID: 37096049 Free PMC article.
A novel methodology for NETs visualization under light microscopy.
Muñiz-Buenrostro A, Arce-Mendoza AY, Montes-Zapata EI, Calderón-Meléndez RC, Vaquera-Alfaro HA, Huerta-Polina JA, Montelongo-Rodríguez MJ. Muñiz-Buenrostro A, et al. Biochem Biophys Rep. 2023 Feb 6;34:101437. doi: 10.1016/j.bbrep.2023.101437. eCollection 2023 Jul. Biochem Biophys Rep. 2023. PMID: 36817094 Free PMC article.

References

1. Brinkmann V (2004) Neutrophil extracellular traps kill bacteria. Science 303:1532–1535. https://doi.org/10.1126/science.1092385 - DOI - PubMed
1. Yousefi S, Mihalache C, Kozlowski E et al (2009) Viable neutrophils release mitochondrial DNA to form neutrophil extracellular traps. Cell Death Differ 16:1438–1444. https://doi.org/10.1038/cdd.2009.96 - DOI - PubMed
1. Boeltz S, Amini P, Anders HJ et al (2019) To NET or not to NET:current opinions and state of the science regarding the formation of neutrophil extracellular traps. Cell Death Differ 26:395–408. https://doi.org/10.1038/s41418-018-0261-x - DOI - PubMed - PMC
1. Knight JS, Carmona-Rivera C, Kaplan MJ (2012) Proteins derived from neutrophil extracellular traps may serve as self-antigens and mediate organ damage in autoimmune diseases. Front Immunol 3:1–12. https://doi.org/10.3389/fimmu.2012.00380 - DOI
1. Fuchs TA, Abed U, Goosmann C et al (2007) Novel cell death program leads to neutrophil extracellular traps. J Cell Biol 176:231–241. https://doi.org/10.1083/jcb.200606027 - DOI - PubMed - PMC

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
- Springer
Research Materials
- NCI CPTC Antibody Characterization Program

[1] Brinkmann V (2004) Neutrophil extracellular traps kill bacteria. Science 303:1532–1535. https://doi.org/10.1126/science.1092385 - DOI - PubMed

[2] Brinkmann V (2004) Neutrophil extracellular traps kill bacteria. Science 303:1532–1535. https://doi.org/10.1126/science.1092385 - DOI - PubMed

[3] Yousefi S, Mihalache C, Kozlowski E et al (2009) Viable neutrophils release mitochondrial DNA to form neutrophil extracellular traps. Cell Death Differ 16:1438–1444. https://doi.org/10.1038/cdd.2009.96 - DOI - PubMed

[4] Yousefi S, Mihalache C, Kozlowski E et al (2009) Viable neutrophils release mitochondrial DNA to form neutrophil extracellular traps. Cell Death Differ 16:1438–1444. https://doi.org/10.1038/cdd.2009.96 - DOI - PubMed

[5] Boeltz S, Amini P, Anders HJ et al (2019) To NET or not to NET:current opinions and state of the science regarding the formation of neutrophil extracellular traps. Cell Death Differ 26:395–408. https://doi.org/10.1038/s41418-018-0261-x - DOI - PubMed - PMC

[6] Boeltz S, Amini P, Anders HJ et al (2019) To NET or not to NET:current opinions and state of the science regarding the formation of neutrophil extracellular traps. Cell Death Differ 26:395–408. https://doi.org/10.1038/s41418-018-0261-x - DOI - PubMed - PMC

[7] Knight JS, Carmona-Rivera C, Kaplan MJ (2012) Proteins derived from neutrophil extracellular traps may serve as self-antigens and mediate organ damage in autoimmune diseases. Front Immunol 3:1–12. https://doi.org/10.3389/fimmu.2012.00380 - DOI

[8] Knight JS, Carmona-Rivera C, Kaplan MJ (2012) Proteins derived from neutrophil extracellular traps may serve as self-antigens and mediate organ damage in autoimmune diseases. Front Immunol 3:1–12. https://doi.org/10.3389/fimmu.2012.00380 - DOI

[9] Fuchs TA, Abed U, Goosmann C et al (2007) Novel cell death program leads to neutrophil extracellular traps. J Cell Biol 176:231–241. https://doi.org/10.1083/jcb.200606027 - DOI - PubMed - PMC

[10] Fuchs TA, Abed U, Goosmann C et al (2007) Novel cell death program leads to neutrophil extracellular traps. J Cell Biol 176:231–241. https://doi.org/10.1083/jcb.200606027 - DOI - PubMed - PMC

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps

Affiliations

In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps

Authors

Affiliations

Abstract

Similar articles

Cited by

References

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Research Materials

Abstract

Similar articles

Cited by

References

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Research Materials