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. 2021 Jun 15;10(6):1216.
doi: 10.3390/plants10061216.

The Effect of Ascophyllum nodosum Extract on the Nutraceutical Antioxidant Potential of Vigna radiata Sprout under Salt Stress

Sangeeta Kumari  1 , Deepak Phogat  2 , Krishnan D Sehrawat  3 , Ravish Choudhary  4 , Vishnu D Rajput  5 , Jyoti Ahlawat  1 , Rohini Karunakaran  6 , Tatiana Minkina  5 , Anita R Sehrawat  1
Affiliations

The Effect of Ascophyllum nodosum Extract on the Nutraceutical Antioxidant Potential of Vigna radiata Sprout under Salt Stress

Sangeeta Kumari et al. Plants (Basel). .

Abstract

Mung bean (Vigna radiata L.) sprout is a popular fresh vegetable, tasty and high in antioxidants. To increase yield and quality after the occurrence of both abiotic and biotic stresses, the application of seaweed extracts is of great importance. Hence, this study was conducted to determine the effect of Ascophyllum nodosum extract (ANE) in the presence of salt on the antioxidant potential of V. radiata sprouts. Different concentrations of ANE viz. 0.00, 0.01, 0.05, 0.10, and 0.50% and NaCl 0, 25, 50, 75, and 100 mM alone and in combinations were tested for researching the antioxidant potential of V. radiata sprouts at 0, 24, and 36 h of sprouting. The DPPH free-radical-scavenging activity of sprouts of V. radiata was found to increase with time and peaked at 24 h of treatment. The A. nodosum extract (0.01%) could reverse the ill effect of the low level of salinity posed by up to 25 mM NaCl. The increasing salinity deteriorated the antioxidant activity using ABTS method of sprouts down to 20.45% of the control at 100 mM NaCl. The total phenolic content (TPC), total flavonoid content (TFC), and reducing power of V. radiata sprouts was found to increase till 36 h of sprouting. A slight increase in TPC, TFC and reducing power was observed when seeds were treated with low concentrations of ANE. The elevation in TPC, TFC and reducing power upon treatment with low concentrations of ANE was also noticed in sprouts in saline combinations. Alpha amylase inhibition activity was found to reach a (67.16% ± 0.9) maximum at 24 h of sprouting at a 0.01% concentration of ANE. Tyrosinase inhibition and alpha glucosidase inhibition was 88.0% ± 2.11 and 84.92% ± 1.2 at 36 h of sprouting, respectively, at 0.01% concentration of ANE. A. nodosum extract is natural, environmentally friendly, and safe, and could be used as one of the strategies to decline stress at a low level and enhance the antioxidant activities in V. radiata sprouts, thus increasing its potential to be developed as an antioxidant-based functional food.

Keywords: Ascophyllum nodosum; antioxidants; germination; salinity; sprouts.

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Conflict of interest statement

The authors declare there are no competing interests.

Figures

Figure 1
Figure 1
DPPH and ABTS radical-scavenging activity (% inhibition) of sprouts of Vigna radiata under salt stress in the presence of A. nodosum extract (ANE). (A0–A4 are ANE concentrations of 0.00, 0.01, 0.05, 0.10, and 0.50%, and T0–T4 are NaCl concentrations of 0, 25, 50, 75 and 100 mM, respectively). Bar indicates mean ± SD (n = 3).
Figure 2
Figure 2
Phenolic (µg GAE) and total flavonoid contents (µg QE) in sprouts of V. radiata under salt stress in the presence of A. nodosum Extract (ANE). (A0–A4 are ANE concentrations 0.00, 0.01, 0.05, 0.10, and 0.50% and T0–T4 are NaCl concentrations 0, 25, 50, 75 and 100 mM, respectively). Bar indicates mean ± SD (n = 3).
Figure 3
Figure 3
Reducing power assay and alpha amylase activity of sprouts of V. radiata under salt stress in the presence of A. nodosum Extract (ANE). (A0–A4 are ANE concentrations 0.00, 0.01, 0.05, 0.10, and 0.50% and T0–T4 are NaCl concentrations 0, 25, 50, 75, and 100 mM, respectively). Bar indicates mean ± SD (n = 3).
Figure 4
Figure 4
Alpha-glucosidase and tyrosinase inhibition of sprouts of V. radiata under salt stress in the presence of A. nodosum extract (ANE). (A0–A4 are ANE concentrations 0.00, 0.01, 0.05, 0.10, and 0.50% and T0–T4 are NaCl concentrations 0, 25, 50, 75 and 100 Mm, respectively). Bar indicates mean ± SD (n = 3).
Figure 5
Figure 5
Standard curve of Gallic acid.
Figure 6
Figure 6
Standard curve of Quercetin.
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