doi: 10.1016/0006-291x(87)90659-0.
Specific A . T DNA sequence binding of RP-HPLC purified HMG-I
- PMID: 3470007
- DOI: 10.1016/0006-291x(87)90659-0
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Specific A . T DNA sequence binding of RP-HPLC purified HMG-I
Biochem Biophys Res Commun.
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Abstract
HMG-I (alpha-protein) is a high mobility group protein which recognizes and binds specifically to A . T rich double stranded DNA. We have investigated, by electrophoretic shift assays and DNase I footprinting, the ability of reverse-phase high performance liquid chromatography purified HMG-I to bind to specific A . T rich duplex DNA sequences. We show here that when HMG-I is isolated and purified under denaturing conditions it retains its specific A . T DNA binding activity. These results suggest that reverse-phase high performance liquid chromatography to be the method of choice for the preparation of HMG-I.
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