doi: 10.1126/science.3685975.
Purification and properties of Drosophila heat shock activator protein
Affiliations
- PMID: 3685975
- DOI: 10.1126/science.3685975
Item in Clipboard
Purification and properties of Drosophila heat shock activator protein
Science.
.
Abstract
Drosophila heat shock activator protein, a rare transacting factor which is induced upon heat shock to bind specifically to the heat shock regulatory sequence in vivo, has been purified from shocked cells to more than 95 percent homogeneity by sequence-specific duplex oligonucleotide affinity chromatography. The purified protein has a relative molecular mass of 110 kilodaltons, binds to the regulatory sequence with great affinity and specificity, and strongly stimulates transcription of the Drosophila hsp70 gene. Studies with this regulatory protein should lead to an understanding of the biochemical pathway underlying the heat shock phenomenon.
Similar articles
-
A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.Cell. 1984 May;37(1):273-83. doi: 10.1016/0092-8674(84)90323-4. Cell. 1984. PMID: 6722872
-
Induction of sequence-specific binding of Drosophila heat shock activator protein without protein synthesis.Nature. 1987 Jun 25-Jul 1;327(6124):727-30. doi: 10.1038/327727a0. Nature. 1987. PMID: 3600771
-
Activating protein factor binds in vitro to upstream control sequences in heat shock gene chromatin.Nature. 1984 Sep 6-11;311(5981):81-4. doi: 10.1038/311081a0. Nature. 1984. PMID: 6472466
-
[Structure and expression of genes of the heat-shock gene family hsp70].Postepy Biochem. 1991;37(2):62-9. Postepy Biochem. 1991. PMID: 1819796 Review. Polish. No abstract available.
-
Heat shock response in Drosophila.Experientia. 1992 Jul 15;48(7):623-9. doi: 10.1007/BF02118306. Experientia. 1992. PMID: 1639169 Review.
Cited by
-
USF-related transcription factor, HIV-TF1, stimulates transcription of human immunodeficiency virus-1.Nucleic Acids Res. 1991 Sep 11;19(17):4689-94. doi: 10.1093/nar/19.17.4689. Nucleic Acids Res. 1991. PMID: 1653950 Free PMC article.
-
Measurement of the binding of transcription factor Sp1 to a single GC box recognition sequence.Nucleic Acids Res. 1989 Apr 11;17(7):2639-53. doi: 10.1093/nar/17.7.2639. Nucleic Acids Res. 1989. PMID: 2717405 Free PMC article.
-
Protein/DNA architecture of the DNase I hypersensitive region of the Drosophila hsp26 promoter.EMBO J. 1988 Jul;7(7):2191-201. doi: 10.1002/j.1460-2075.1988.tb03058.x. EMBO J. 1988. PMID: 2901349 Free PMC article.
-
Interaction of cellular factors related to the Jun oncoprotein with the promoter of a replication-dependent hamster histone H3.2 gene.Proc Natl Acad Sci U S A. 1989 Jan;86(2):491-5. doi: 10.1073/pnas.86.2.491. Proc Natl Acad Sci U S A. 1989. PMID: 2492109 Free PMC article.
-
Regulation of heat shock factor in Schizosaccharomyces pombe more closely resembles regulation in mammals than in Saccharomyces cerevisiae.Mol Cell Biol. 1991 Jan;11(1):281-8. doi: 10.1128/mcb.11.1.281-288.1991. Mol Cell Biol. 1991. PMID: 1986225 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
