Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Mar 13;31(6):10918-10935.
doi: 10.1364/OE.485699.

Airy beam light sheet microscopy boosted by deep learning deconvolution

Anne Stockhausen, Juan Eduardo Rodriguez-Gatica, Jens Schweihoff, Martin Karl Schwarz, Ulrich Kubitscheck
Free article

Airy beam light sheet microscopy boosted by deep learning deconvolution

Anne Stockhausen et al. Opt Express. .
Free article

Abstract

Common light sheet microscopy comes with a trade-off between light sheet width defining the optical sectioning and the usable field of view arising from the divergence of the illuminating Gaussian beam. To overcome this, low-diverging Airy beams have been introduced. Airy beams, however, exhibit side lobes degrading image contrast. Here, we constructed an Airy beam light sheet microscope, and developed a deep learning image deconvolution to remove the effects of the side lobes without knowledge of the point spread function. Using a generative adversarial network and high-quality training data, we significantly enhanced image contrast and improved the performance of a bicubic upscaling. We evaluated the performance with fluorescently labeled neurons in mouse brain tissue samples. We found that deep learning-based deconvolution was about 20-fold faster than the standard approach. The combination of Airy beam light sheet microscopy and deep learning deconvolution allows imaging large volumes rapidly and with high quality.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

LinkOut - more resources

  NODES
3d 1
HOME 2
Javascript 1
os 24
text 12
Training 1
twitter 2
web 4