High-throughput volumetric mapping of synaptic transmission

doi:10.1038/s41592-024-02309-3

. 2024 Jul;21(7):1298-1305.

doi: 10.1038/s41592-024-02309-3. Epub 2024 Jun 19.

High-throughput volumetric mapping of synaptic transmission

Wei Chen^{1

2}, Xinxin Ge^{3

4}, Qinrong Zhang¹, Ryan G Natan^{1

5}, Jiang Lan Fan^{6

7}, Massimo Scanziani^{3

4}, Na Ji^{8

9

10

11}

Affiliations

¹ Department of Physics, University of California, Berkeley, CA, USA.
² School of Mechanical Science and Engineering - Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, Wuhan, China.
³ Department of Physiology, University of California, San Francisco, San Francisco, CA, USA.
⁴ Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.
⁵ Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
⁶ Joint Bioengineering Graduate Program, University of California, Berkeley, CA, USA.
⁷ Joint Bioengineering Graduate Program, University of California, San Francisco, CA, USA.
⁸ Department of Physics, University of California, Berkeley, CA, USA. jina@berkeley.edu.
⁹ Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. jina@berkeley.edu.
¹⁰ Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA. jina@berkeley.edu.
¹¹ Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. jina@berkeley.edu.

PMID: 38898094
DOI: 10.1038/s41592-024-02309-3

High-throughput volumetric mapping of synaptic transmission

Wei Chen et al. Nat Methods. 2024 Jul.

. 2024 Jul;21(7):1298-1305.

doi: 10.1038/s41592-024-02309-3. Epub 2024 Jun 19.

Authors

Wei Chen^{1

2}, Xinxin Ge^{3

4}, Qinrong Zhang¹, Ryan G Natan^{1

5}, Jiang Lan Fan^{6

7}, Massimo Scanziani^{3

4}, Na Ji^{8

9

10

11}

Affiliations

¹ Department of Physics, University of California, Berkeley, CA, USA.
² School of Mechanical Science and Engineering - Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, Wuhan, China.
³ Department of Physiology, University of California, San Francisco, San Francisco, CA, USA.
⁴ Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, USA.
⁵ Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
⁶ Joint Bioengineering Graduate Program, University of California, Berkeley, CA, USA.
⁷ Joint Bioengineering Graduate Program, University of California, San Francisco, CA, USA.
⁸ Department of Physics, University of California, Berkeley, CA, USA. jina@berkeley.edu.
⁹ Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. jina@berkeley.edu.
¹⁰ Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA. jina@berkeley.edu.
¹¹ Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. jina@berkeley.edu.

PMID: 38898094
DOI: 10.1038/s41592-024-02309-3

Abstract

Volumetric imaging of synaptic transmission in vivo requires high spatial and high temporal resolution. Shaping the wavefront of two-photon fluorescence excitation light, we developed Bessel-droplet foci for high-contrast and high-resolution volumetric imaging of synapses. Applying our method to imaging glutamate release, we demonstrated high-throughput mapping of excitatory inputs at >1,000 synapses per volume and >500 dendritic spines per neuron in vivo and unveiled previously unseen features of functional synaptic organization in the mouse primary visual cortex.

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Adaptive optical correction for in vivo two-photon fluorescence microscopy with neural fields.
Kang I, Kim H, Natan R, Zhang Q, Yu SX, Ji N. Kang I, et al. bioRxiv [Preprint]. 2024 Dec 6:2024.10.20.619284. doi: 10.1101/2024.10.20.619284. bioRxiv. 2024. PMID: 39484436 Free PMC article. Preprint.
Multiphoton fluorescence microscopy for in vivo imaging.
Xu C, Nedergaard M, Fowell DJ, Friedl P, Ji N. Xu C, et al. Cell. 2024 Aug 22;187(17):4458-4487. doi: 10.1016/j.cell.2024.07.036. Cell. 2024. PMID: 39178829 Review.

References

1. Denk, W., Strickler, J. H. & Webb, W. W. Two-photon laser scanning fluorescence microscopy. Science 248, 73–76 (1990). - DOI - PubMed
1. Hedrick, N. G. et al. Learning binds new inputs into functional synaptic clusters via spinogenesis. Nat. Neurosci. 25, 726–737 (2022). - DOI - PubMed
1. Grienberger, C. & Konnerth, A. Imaging Calcium in Neurons. Neuron 73, 862–885 (2012). - DOI - PubMed
1. Kerlin, A. et al. Functional clustering of dendritic activity during decision-making. eLife 8, e46966 (2019). - DOI - PubMed - PMC
1. London, M. & Häusser, M. Dendritic computation. Annu. Rev. Neurosci. 28, 503–532 (2005). - DOI - PubMed

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U19NS107613/U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS)

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[1] Denk, W., Strickler, J. H. & Webb, W. W. Two-photon laser scanning fluorescence microscopy. Science 248, 73–76 (1990). - DOI - PubMed

[2] Denk, W., Strickler, J. H. & Webb, W. W. Two-photon laser scanning fluorescence microscopy. Science 248, 73–76 (1990). - DOI - PubMed

[3] Hedrick, N. G. et al. Learning binds new inputs into functional synaptic clusters via spinogenesis. Nat. Neurosci. 25, 726–737 (2022). - DOI - PubMed

[4] Hedrick, N. G. et al. Learning binds new inputs into functional synaptic clusters via spinogenesis. Nat. Neurosci. 25, 726–737 (2022). - DOI - PubMed

[5] Grienberger, C. & Konnerth, A. Imaging Calcium in Neurons. Neuron 73, 862–885 (2012). - DOI - PubMed

[6] Grienberger, C. & Konnerth, A. Imaging Calcium in Neurons. Neuron 73, 862–885 (2012). - DOI - PubMed

[7] Kerlin, A. et al. Functional clustering of dendritic activity during decision-making. eLife 8, e46966 (2019). - DOI - PubMed - PMC

[8] Kerlin, A. et al. Functional clustering of dendritic activity during decision-making. eLife 8, e46966 (2019). - DOI - PubMed - PMC

[9] London, M. & Häusser, M. Dendritic computation. Annu. Rev. Neurosci. 28, 503–532 (2005). - DOI - PubMed

[10] London, M. & Häusser, M. Dendritic computation. Annu. Rev. Neurosci. 28, 503–532 (2005). - DOI - PubMed

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High-throughput volumetric mapping of synaptic transmission

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High-throughput volumetric mapping of synaptic transmission

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