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. 2024 Dec;19(1):2375030.
doi: 10.1080/15592294.2024.2375030. Epub 2024 Jul 5.

Combined exercise training decreases blood pressure in OLDER women with NOS3 polymorphism providing changes in differentially methylated regions (DMRs)

Guilherme da Silva Rodrigues  1 , Natalia Yumi Noronha  2 , João Gabriel Ribeiro de Lima  1 , Isabela Harumi Yonehara Noma  3 , Andressa Crystine da Silva Sobrinho  1 , Luísa Maria Diani  1 , Ana P Pinto  4 , Karine Pereira Rodrigues  1 , Marcela Augusta de Souza Pinhel  5 , Carla Barbosa Nonino  5 , Lígia Moriguchi Watanabe  5 , Carlos Roberto Bueno Júnior  1   4
Affiliations

Combined exercise training decreases blood pressure in OLDER women with NOS3 polymorphism providing changes in differentially methylated regions (DMRs)

Guilherme da Silva Rodrigues et al. Epigenetics. 2024 Dec.

Abstract

The mechanisms by which the ageing process is associated to an unhealthy lifestyle and how they play an essential role in the aetiology of systemic arterial hypertension have not yet been completely elucidated. Our objective is to investigate the influence of NOS3 polymorphisms [-786T > C and (Glu298Asp)] on systolic blood pressure (SBP) and diastolic blood pressure (DBP) response, differentially methylated regions (DMRs), and physical fitness of adult and older women after a 14-week combined training intervention. The combined training was carried out for 14 weeks, performed 3 times a week, totalling 180 minutes weekly. The genotyping experiment used Illumina Infinium Global Screening Array version 2.0 (GSA V2.0) and Illumina's EPIC Infinium Methylation BeadChip. The participants were separated into SNP rs2070744 in TT (59.7 ± 6.2 years) and TC + CC (60.0 ± 5.2 years), and SNP rs17999 in GluGlu (58.8 ± 5.7 years) and GluAsp + AspAsp (61.6 ± 4.9 years). We observed an effect of time for variables BP, physical capacities, and cholesterol. DMRs related to SBP and DBP were identified for the rs2070744 and rs17999 groups pre- and decreased numbers of DMRs post-training. When we analysed the effect of exercise training in pre- and post-comparisons, the GluGlu SNP (rs17999) showed 10 DMRs, and after enrichment, we identified several biological biases. The combined training improved the SBP and DBP values of the participants regardless of the SNPs. In addition, exercise training affected DNA methylation differently between the groups of NOS3 polymorphisms.

Keywords: DNA methylation; Physical exercise; epigenetics; women.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Flowchart.
Figure 2.
Figure 2.
Individual changes in systolic and diastolic blood pressure values after 14 weeks of combined exercise training.
Figure 3.
Figure 3.
Differentially methylated regions associated with systolic and diastolic blood pressure for the SNPs TC+CC and TT.
Figure 4.
Figure 4.
KEEG enrichment of DMR sites for TC+CC and TT SNPs.
Figure 5.
Figure 5.
Differentially methylated regions related to systolic and diastolic blood pressure for the SNPs GluAsp+AspAsp and GluGlu.
Figure 6.
Figure 6.
KEEG enrichment of DMR sites for TC+CC and TT SNPs.
Figure 7.
Figure 7.
Enrichment of DMRs for the GluGlu SNP after 14 weeks of combined training.
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Grants and funding

This work was supported in part by a grant from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (23/08249-0).
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