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. 2024 Nov-Dec;14(6):815-824.
doi: 10.1016/j.jobcr.2024.10.014. Epub 2024 Nov 4.

Antimicrobial role of Lavandula angustifolia towards Candida albicans, Streptococcus mutans, Staphylococcus aureus and anti-adherence effect on denture base resin

Anitha Kuttae Viswanathan  1 , Rajkumar Krishnan  2
Affiliations

Antimicrobial role of Lavandula angustifolia towards Candida albicans, Streptococcus mutans, Staphylococcus aureus and anti-adherence effect on denture base resin

Anitha Kuttae Viswanathan et al. J Oral Biol Craniofac Res. 2024 Nov-Dec.

Abstract

Objectives: The objective of the study was to determine the antimicrobial efficacy of Lavandula angustifolia (True Lavender extract) towards Candida albicans, Streptococcus mutans, Staphylococcus aureus pathogens. Varying proportions of the extract was incorporated into polymethyl methacrylate (PMMA) denture base resin and the anti-adherent effect was investigated.

Methods: An in-vitro study was performed after pure extract was obtained from Lavandula angustifolia (LA) flowers using a solvent based hot extraction process. Chromatographic analysis and computational molecular docking were done to analyze its phytoconstituents with potential _target-ligand bond. Tests of antimicrobial susceptibility, minimum inhibitory concentration, minimum bactericidal and fungicidal dosages and in-vitro cytotoxicity were performed. Different proportions of LA extract (0, .5, 1.0, 1.5, and 2.0 %) were added to PMMA resin to assess anti-adherence property. Obtained data were statistically analyzed with One-way ANOVA followed by Tukey post-hoc tests.

Results: The reports revealed significant antimicrobial susceptibility against the test pathogens compared to control drugs (P > 0.05). A minimum concentration of .02 mg of lavender extract inhibited microbial growth with low cytotoxicity (P < 0.05). The highest anti-adherent activity was observed in the .5 % LA-incorporated PMMA resin group (P = 0.0001).

Conclusions: Incorporating lavender extract into denture base resin demonstrated promising antimicrobial properties. This investigation encourages further research to understand its effects on mechanical and physical properties of reinforced dental resins.

Keywords: Antimicrobial; Denture stomatitis; Edentulism; Lavender extract; Polymethyl methacrylate denture base resin.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Image 1
Graphical abstract
Fig. 1a
Fig. 1a
Comparison of bacteriostatic potential of LA extract against positive control synthetic drugs.
Fig. 1b
Fig. 1b
Measurement of zone of inhibition to determine antimicrobial susceptibility of Lavandula angustifolia extract.
Fig. 2a
Fig. 2a
Serial dilution test for determination of the Minimum inhibitory concentration assay using 96-well microtiter plate.
Fig. 2b
Fig. 2b
Investigation of minimum inhibitory concentration of Lavandula angustifolia extract against S. mutans, S. aureus and C. albicans.
Fig. 3
Fig. 3
In-vitro cytotoxicity effect of Lavandula angustifolia extract at various dilutions.
Fig. 4
Fig. 4
Phytochemical analysis of Lavandula angustifolia extract with chromatogram.
Fig. 5
Fig. 5
2D and 3D interaction between Glucansucrase (GSase) from S. mutans (PDB ID:3 AIB) and camphor from lavender.
Fig. 6
Fig. 6
2D and 3D interaction between cytochrome P450 lanosterol 14 alpha-demethylase from C. albicans (PDB ID: 5V5Z) and 1,8-cineole from lavender.
Fig. 7
Fig. 7
2D and 3D interaction between Dihydrofolate Reductase (DHFR) from S. aureus (PDB ID: 4LAG) and Terpinenol-4 from lavender.
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