The effect of ethidium bromide on mitochondrial DNA synthesis and mitochondrial DNA structure in HeLa cells
- PMID: 5111872
- PMCID: PMC2108249
- DOI: 10.1083/jcb.51.1.116
The effect of ethidium bromide on mitochondrial DNA synthesis and mitochondrial DNA structure in HeLa cells
Abstract
The synthesis of mitochondrial DNA (mDNA) in HeLa cells is selectively inhibited by relatively low concentrations of ethidium bromide. After exposure of cells to strongly inhibitory concentrations of the drug, the apparent superhelix density of mDNA is rapidly increased, as judged by its buoyant density in CsCl in the presence of ethidium bromide. Mitochondrial DNA synthesized in the presence of partially inhibitory concentrations of ethidium bromide is also altered in its buoyant density in the presence of the dye, but is more heterogeneous in this respect. However, the change in buoyant density of newly synthesized mDNA may be explained by changes in structure other than a change in superhelix density, as indicated by its increased resistance to digestion by pancreatic DNase.
Similar articles
-
Differential effect of ethidium bromide and cytosine arabinoside on mitochondrial and nuclear DNA synthesis in HeLa cells.Z Naturforsch B Anorg Chem Org Chem Biochem Biophys Biol. 1972 Aug;27(8):989-91. doi: 10.1515/znb-1972-0826. Z Naturforsch B Anorg Chem Org Chem Biochem Biophys Biol. 1972. PMID: 4404714 No abstract available.
-
Differential effects of ethidium bromide on mitochondrial and nuclear DNA synthesis in vivo in cultured mammalian cells.Exp Cell Res. 1972 May;72(1):211-22. doi: 10.1016/0014-4827(72)90583-6. Exp Cell Res. 1972. PMID: 4337144 No abstract available.
-
Effect of ethidium bromide on mitochondrial DNA and cytochrome synthesis in HeLa cells.Exp Cell Res. 1971 Jun;66(2):410-6. doi: 10.1016/0014-4827(71)90695-1. Exp Cell Res. 1971. PMID: 5556428 No abstract available.
-
Mitochondrial RNA synthesis during mitosis.Science. 1970 Apr 3;168(3927):135-8. doi: 10.1126/science.168.3927.135. Science. 1970. PMID: 5417056
-
Mitochondria-associated ribonucleic acid of the HeLa cell. Effect of ethidium bromide on the synthesis of ribosomal and 4S ribonucleic acid.Biochemistry. 1969 Dec;8(12):5089-93. doi: 10.1021/bi00840a062. Biochemistry. 1969. PMID: 5365795 No abstract available.
Cited by
-
Integrity of the yeast mitochondrial genome, but not its distribution and inheritance, relies on mitochondrial fission and fusion.Proc Natl Acad Sci U S A. 2015 Mar 3;112(9):E947-56. doi: 10.1073/pnas.1501737112. Epub 2015 Feb 17. Proc Natl Acad Sci U S A. 2015. PMID: 25730886 Free PMC article.
-
LRRK2 maintains mitochondrial homeostasis and regulates innate immune responses to Mycobacterium tuberculosis.Elife. 2020 Feb 14;9:e51071. doi: 10.7554/eLife.51071. Elife. 2020. PMID: 32057291 Free PMC article.
-
RAD51C/XRCC3 Facilitates Mitochondrial DNA Replication and Maintains Integrity of the Mitochondrial Genome.Mol Cell Biol. 2018 Jan 16;38(3):e00489-17. doi: 10.1128/MCB.00489-17. Print 2018 Feb 1. Mol Cell Biol. 2018. PMID: 29158291 Free PMC article.
-
Loss of hif-1 promotes resistance to the exogenous mitochondrial stressor ethidium bromide in Caenorhabditis elegans.BMC Cell Biol. 2016 Sep 13;17 Suppl 1(1):34. doi: 10.1186/s12860-016-0112-x. BMC Cell Biol. 2016. PMID: 27618966 Free PMC article.
-
MRE11-dependent instability in mitochondrial DNA fork protection activates a cGAS immune signaling pathway.Sci Adv. 2021 Dec 17;7(51):eabf9441. doi: 10.1126/sciadv.abf9441. Epub 2021 Dec 15. Sci Adv. 2021. PMID: 34910513 Free PMC article.
