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. 1982 Feb;79(4):1245-9.
doi: 10.1073/pnas.79.4.1245.

Unique glycoprotein-proteoglycan complex defined by monoclonal antibody on human melanoma cells

T F Bumol, R A Reisfeld

Unique glycoprotein-proteoglycan complex defined by monoclonal antibody on human melanoma cells

T F Bumol et al. Proc Natl Acad Sci U S A. 1982 Feb.

Abstract

A monoclonal antibody, 9.2.27, with a high specificity for human melanoma cell surfaces has been utilized for biosynthetic studies in M21 human melanoma cells to define a unique antigenic complex consisting of a 250-kilodalton N-linked glycoprotein and a high molecular weight proteoglycan component larger than 400 kilodaltons. The 250-kilodalton glycoprotein has endoglycosidase H-sensitive precursors and shows a lower apparent molecular weight after treatment with neuraminidase. The biosynthesis of the proteoglycan component is inhibited by exposure of M21 cells to the monovalent ionophore monensin, this component can be labeled biosynthetically with 35SO4, is sensitive to beta-elimination in dilute base, and is degraded by both chondroitinase AC and ABC lyases, suggesting that it is a chondroitin sulfate proteoglycan. These data demonstrate that the antigenic determinant recognized by monoclonal antibody 9.2.27 is located on a glycoprotein-proteoglycan complex which may have unique implications for the interaction of glycoconjugates at the human melanoma tumor cell surface.

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