Establishment of a new peroxidase-positive human myeloid cell line, PL-21
- PMID: 6362749
Establishment of a new peroxidase-positive human myeloid cell line, PL-21
Abstract
A myeloid cell line, designated PL-21, was established from the peripheral blood of a patient with acute promyelocytic leukemia. The PL-21 cell line grew in single-cell suspension, with a doubling time of 48-64 hr, and consisted of promyelocytes with fine immature nuclei and prominent azurophilic granules in the cytoplasm. PL-21 cells were positive for peroxidase, naphthol AS-D chloroacetate esterase, and Sudan Black B staining. Under the usual culture conditions, a small proportion of these cells differentiated into mature granulocytes, and this differentiation was enhanced by the addition of dimethyl sulfoxide in the culture medium. PL-21 cells had receptors for the Fc portion of IgG and complement, intracytoplasmic lysozyme and phagocytic activity, but lacked Epstein-Barr virus-associated nuclear antigen. Chromosome analysis of this cell line revealed a human male polyploid karyotype with 13q+ and double minute chromosomes. This new myeloid cell line may provide useful material for the study of proliferation and differentiation of human leukemia cells.
Similar articles
-
Two new human myeloid cell lines derived from acute promyelocytic leukemia and chronic myelocytic leukemia.Gan. 1983 Jun;74(3):319-22. Gan. 1983. PMID: 6576961
-
Establishment of a new myeloid leukemia cell line (TK-1), and isolation of cells having a translocation involving a band 17q23.Int J Cancer. 1986 May 15;37(5):761-7. doi: 10.1002/ijc.2910370518. Int J Cancer. 1986. PMID: 3457771
-
Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome.Blood. 1985 Dec;66(6):1384-92. Blood. 1985. PMID: 2998511
-
Establishment and characterization of a human myeloid cell line from Philadelphia chromosome-negative myeloblastic leukemia arising in a patient with myelodysplastic syndrome.Blood. 1987 Nov;70(5):1665-72. Blood. 1987. PMID: 3478106
-
Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications.Blood. 1983 Oct;62(4):709-21. Blood. 1983. PMID: 6192859 Review.
Cited by
-
A monoclonal antibody that recognizes a common antigen in myeloid and human T-cell leukemia virus type I-carrying cells.Jpn J Cancer Res. 1988 Jul;79(7):809-12. doi: 10.1111/j.1349-7006.1988.tb00040.x. Jpn J Cancer Res. 1988. PMID: 3139595 Free PMC article.
-
Potentiation of growth-inhibitory activity of 9-beta-D-arabinofuranosyladenine by 2'-deoxycoformycin in human cultured cell lines derived from leukemias and lymphomas.Jpn J Cancer Res. 1989 May;80(5):482-9. doi: 10.1111/j.1349-7006.1989.tb02340.x. Jpn J Cancer Res. 1989. PMID: 2502523 Free PMC article.
-
MicroRNA-150 expression induces myeloid differentiation of human acute leukemia cells and normal hematopoietic progenitors.PLoS One. 2013 Sep 24;8(9):e75815. doi: 10.1371/journal.pone.0075815. eCollection 2013. PLoS One. 2013. PMID: 24086639 Free PMC article.
-
Multidrug resistance in cultured human leukemia and lymphoma cell lines detected by a monoclonal antibody, MRK16.Jpn J Cancer Res. 1989 Oct;80(10):1006-13. doi: 10.1111/j.1349-7006.1989.tb01641.x. Jpn J Cancer Res. 1989. PMID: 2575608 Free PMC article.
-
Electron microscopic and immunohistochemical observations of differentiation of human myeloid leukemia line, PL-21.J Cancer Res Clin Oncol. 1986;111(3):203-8. doi: 10.1007/BF00389235. J Cancer Res Clin Oncol. 1986. PMID: 3460994
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
